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dc.contributor.authorGomes-Alves, Patrícia
dc.contributor.authorSerra, Margarida
dc.contributor.authorBrito, Catarina
dc.contributor.authorR-Borlado, Luis
dc.contributor.authorLopez, Juan Antonio 
dc.contributor.authorVazquez, Jesus 
dc.contributor.authorCarrondo, Manuel J T
dc.contributor.authorBernad, Antonio 
dc.contributor.authorAlves, Paula M
dc.date.accessioned2018-11-21T14:59:06Z
dc.date.available2018-11-21T14:59:06Z
dc.date.issued2015-04
dc.identifier.citationProteomics, 2015. 15(7): p. 1332-7es_ES
dc.identifier.issn1615-9853es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/6659
dc.description.abstractHuman cardiac stem cells (hCSC) express a portfolio of plasma membrane receptors that are involved in the regulatory auto/paracrine feedback loop mechanism of activation of these cells, and consequently contribute to myocardial regeneration. In order to attain a comprehensive description of hCSC receptome and overcoming the inability demonstrated by other technologies applied in receptor identification, mainly due to the transmembrane nature, high hydrophobic character and relative low concentration of these proteins, we have exploited and improved a proteomics workflow. This approach was based on the enrichment of hCSC plasma membrane fraction and addition of prefractionation steps prior to MS analysis. More than 100 plasma membrane receptors were identified. The data reported herein constitute a valuable source of information to further understand cardiac stem cells activation mechanisms and the subsequent cardiac repair process. All MS data have been deposited in the ProteomeXchange with identifier PXD001117 (http://proteomecentral.proteomexchange.org/dataset/PXD001117).es_ES
dc.description.sponsorshipAuthors acknowledge FP7 EU project CARE-MI (HEALTH-2009_242038) and the Portuguese Foundation for Science and Technology (PTDC/BBBBIO/1414) for financial support. PGA is a recipient of the FCT fellowship SFRH/BPD/86513/2012. MALDI-TOF/TOF analyses were performed at the Mass Spectrometry Unit (UniMS), ITQB/iBET, Oeiras, Portugal. The data deposition to the ProteomeXchange Consortium was supported by PRIDETeam, EBI.
dc.language.isoenges_ES
dc.type.hasVersionAMes_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectBiomedicinees_ES
dc.subjectHuman cardiac stem cellses_ES
dc.subjectNano-LC-MS/MSes_ES
dc.subjectPrefractionationes_ES
dc.subjectReceptomees_ES
dc.subject.meshAdult Stem Cells es_ES
dc.subject.meshCells, Cultured es_ES
dc.subject.meshChromatography, Ion Exchange es_ES
dc.subject.meshHumans es_ES
dc.subject.meshMyocardial Infarction es_ES
dc.subject.meshMyocardium es_ES
dc.subject.meshProteome es_ES
dc.subject.meshProteomics es_ES
dc.subject.meshReceptors, Cell Surfacees_ES
dc.subject.meshRegeneration es_ES
dc.subject.meshTandem Mass Spectrometry es_ES
dc.titleExploring analytical proteomics platforms toward the definition of human cardiac stem cells receptomees_ES
dc.typejournal articlees_ES
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.identifier.pubmedID25504917es_ES
dc.format.volume15es_ES
dc.format.number7es_ES
dc.format.page1332-7es_ES
dc.identifier.doi10.1002/pmic.201400318es_ES
dc.contributor.funderEuropean Commission 
dc.description.peerreviewedes_ES
dc.identifier.e-issn1615-9861es_ES
dc.relation.publisherversionhttps://doi.org/10.1002/pmic.201400318
dc.identifier.journalProteomicses_ES
dc.repisalud.orgCNICCNIC::Unidades técnicas::Proteómica / Metabolómicaes_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Proteómica cardiovasculares_ES
dc.repisalud.institucionCNICes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/242038/EUes_ES
dc.rights.accessRightsopen accesses_ES


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Este Item está sujeto a una licencia Creative Commons: Attribution-NonCommercial-NoDerivatives 4.0 Internacional