Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/6659
Título
Exploring analytical proteomics platforms toward the definition of human cardiac stem cells receptome
Autor(es)
Gomes-Alves, Patrícia | Serra, Margarida | Brito, Catarina | R-Borlado, Luis | Lopez, Juan Antonio CNIC | Vazquez, Jesus CNIC | Carrondo, Manuel J T | Bernad, Antonio CNIC | Alves, Paula M
Fecha de publicación
2015-04
Cita
Proteomics, 2015. 15(7): p. 1332-7
Idioma
Inglés
Tipo de documento
journal article
Resumen
Human cardiac stem cells (hCSC) express a portfolio of plasma membrane receptors that are involved in the regulatory auto/paracrine feedback loop mechanism of activation of these cells, and consequently contribute to myocardial regeneration. In order to attain a comprehensive description of hCSC receptome and overcoming the inability demonstrated by other technologies applied in receptor identification, mainly due to the transmembrane nature, high hydrophobic character and relative low concentration of these proteins, we have exploited and improved a proteomics workflow. This approach was based on the enrichment of hCSC plasma membrane fraction and addition of prefractionation steps prior to MS analysis. More than 100 plasma membrane receptors were identified. The data reported herein constitute a valuable source of information to further understand cardiac stem cells activation mechanisms and the subsequent cardiac repair process. All MS data have been deposited in the ProteomeXchange with identifier PXD001117 (http://proteomecentral.proteomexchange.org/dataset/PXD001117).
Palabras clave
MESH
Adult Stem Cells | Cells, Cultured | Chromatography, Ion Exchange | Humans | Myocardial Infarction | Myocardium | Proteome | Proteomics | Receptors, Cell Surface | Regeneration | Tandem Mass Spectrometry
Versión en línea
DOI
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