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dc.contributor.authorGroult, Hugo 
dc.contributor.authorRuiz-Cabello, Jesus 
dc.contributor.authorLechuga-Vieco, Ana V. 
dc.contributor.authorMateo, Jesus 
dc.contributor.authorBenito, Marina 
dc.contributor.authorBilbao, Izaskun 
dc.contributor.authorMartínez-Alcázar, María Paz
dc.contributor.authorLopez, Juan Antonio 
dc.contributor.authorVazquez, Jesus 
dc.contributor.authorHerranz, Fernando
dc.identifier.citationChemistry, 2014. 20(50): p. 16662-71es_ES
dc.description.abstractWe report the synthesis of micellar phosphatidylcholine-coated superparamagnetic iron oxide nanoparticles as a new long circulation contrast agents for magnetic resonance imaging. Oleic acid-coated Fe3 O4 nanoparticles were first prepared through thermal degradation and then encapsulated into small clusters with a phosphatidylcholine coating to obtain hydrophilic nanomicelles. A thorough characterization confirmed the chemical nature of the coating and the excellent colloidal stability of these nanomicelles in aqueous media. Magnetization and relaxivity properties proved their suitability as magnetic resonance imaging (MRI) contrast agent and in vitro cell viability data showed low toxicity. Vascular lifetime and elimination kinetics in the liver were assessed by blood relaxometry and by in vivo MRI in rats and compared with "control" particles prepared with a polyethylene glycol derivative. These micellar particles had a lifetime in blood of more than 10 h, much longer than the control nanoparticles (≈2 h), which is remarkable considering that the coating molecule is a small biocompatible zwitterionic phospholipid. The protein corona was characterized after incubation with rat serum at different times by high-throughput proteomics, showing a higher proportion of bound apolipoproteins and other dysopsonins for the phosphatidylcholine particles. The antibiofouling properties of this corona and its resistance to the adsorption of proteins corroborate the observed enhanced stability and prolonged systemic circulation.es_ES
dc.description.sponsorshipThis study is supported by a grant from FP7 Marie Curie, Pulmonary imaging network (PINET), by Fundacio La Maratode TV3 (70/C/2012) and by a grant from the Comunidad de Madrid (S2010/BMD-2326, Inmunothercan-CM) and by Spanish Economy Ministry (MAT2013-47303-P). We thank E. Urones (Centro Nacional de Microscopia de la Universidad Complutense de Madrid) for the transmission electronic microscopy imaging; P. Morales (Instituto de Ciencia de Materiales de la Universidad Autonoma de Madrid) for the thermogravimetric and magnetization analysis and B. Salinas (Fundacion Centro Nacional de Investigaciones Cardiovasculares and CIBER de Enfermedades Respiratorias) for the TEM picture of oleic acid coated Fe3 O4. The authors declare no competing financial interests.
dc.subjectmagnetic propertieses_ES
dc.subject.meshAnimals es_ES
dc.subject.meshCell Line es_ES
dc.subject.meshContrast Media es_ES
dc.subject.meshFerric Compounds es_ES
dc.subject.meshMagnetic Resonance Imaging es_ES
dc.subject.meshMagnetite Nanoparticles es_ES
dc.subject.meshMale es_ES
dc.subject.meshMice, Inbred C57BL es_ES
dc.subject.meshMicelles es_ES
dc.subject.meshPhosphatidylcholines es_ES
dc.subject.meshRats es_ES
dc.subject.meshRats, Wistar es_ES
dc.titlePhosphatidylcholine-coated iron oxide nanomicelles for in vivo prolonged circulation time with an antibiofouling protein coronaes_ES
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.contributor.funderEuropean Commision
dc.contributor.funderFundacio la Marato
dc.contributor.funderMinisterio de Economía y Competitividad (España)
dc.contributor.funderComunidad de Madrid
dc.identifier.journalChemistry (Weinheim an der Bergstrasse, Germany)es_ES
dc.repisalud.orgCNICCNIC::Unidades técnicas::Proteómica / Metabolómicaes_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Proteómica cardiovasculares_ES
dc.repisalud.orgCNICCNIC::Unidades técnicas

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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
This item is licensed under a: Attribution-NonCommercial-NoDerivatives 4.0 Internacional