dc.contributor.author | Rodriguez, Ariel | |
dc.contributor.author | Falcon, Ana | |
dc.contributor.author | Cuevas, Maria Teresa | |
dc.contributor.author | Pozo Sanchez, Francisco | |
dc.contributor.author | Guerra, Susana | |
dc.contributor.author | Garcia-Barreno, Blanca | |
dc.contributor.author | Martinez-Orellana, Pamela | |
dc.contributor.author | Perez-Breña, Pilar | |
dc.contributor.author | Montoya, Maria | |
dc.contributor.author | Melero, Jose Antonio | |
dc.contributor.author | Pizarro, Manuel | |
dc.contributor.author | Ortin, Juan | |
dc.contributor.author | Casas Flecha, Inmaculada | |
dc.contributor.author | Nieto, Amelia | |
dc.date.accessioned | 2018-11-20T10:47:10Z | |
dc.date.available | 2018-11-20T10:47:10Z | |
dc.date.issued | 2013-01-10 | |
dc.identifier.citation | PLoS One. 2013;8(1):e53515 | es_ES |
dc.identifier.issn | 1932-6203 | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/6637 | |
dc.description.abstract | Pandemic 2009 H1N1 (pH1N1) influenza viruses caused mild symptoms in most infected patients. However, a greater rate of severe disease was observed in healthy young adults and children without co-morbid conditions. Here we tested whether influenza strains displaying differential virulence could be present among circulating pH1N1 viruses. The biological properties and the genotype of viruses isolated from a patient showing mild disease (M) or from a fatal case (F), both without known co-morbid conditions were compared in vitro and in vivo. The F virus presented faster growth kinetics and stronger induction of cytokines than M virus in human alveolar lung epithelial cells. In the murine model in vivo, the F virus showed a stronger morbidity and mortality than M virus. Remarkably, a higher proportion of mice presenting infectious virus in the hearts, was found in F virus-infected animals. Altogether, the data indicate that strains of pH1N1 virus with enhanced pathogenicity circulated during the 2009 pandemic. In addition, examination of chemokine receptor 5 (CCR5) genotype, recently reported as involved in severe influenza virus disease, revealed that the F virus-infected patient was homozygous for the deleted form of CCR5 receptor (CCR5Δ32). | es_ES |
dc.description.sponsorship | Funding Statement: This work was supported by Instituto de Salud Carlos III (Programa especial de investigación sobre la gripe pándemica GR09/0023, GR09/0040, GR09/0039) and Ciber de Enfermedades Respiratorias. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Public Library of Science (PLOS) | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject.mesh | Adult | es_ES |
dc.subject.mesh | Alleles | es_ES |
dc.subject.mesh | Amino Acids | es_ES |
dc.subject.mesh | Animals | es_ES |
dc.subject.mesh | Cell Line | es_ES |
dc.subject.mesh | Cells, Cultured | es_ES |
dc.subject.mesh | Cytokines | es_ES |
dc.subject.mesh | Epithelial Cells | es_ES |
dc.subject.mesh | Female | es_ES |
dc.subject.mesh | Humans | es_ES |
dc.subject.mesh | Immunohistochemistry | es_ES |
dc.subject.mesh | Influenza A Virus, H1N1 Subtype | es_ES |
dc.subject.mesh | Influenza, Human | es_ES |
dc.subject.mesh | Mice | es_ES |
dc.subject.mesh | Mice, Inbred BALB C | es_ES |
dc.subject.mesh | Orthomyxoviridae Infections | es_ES |
dc.subject.mesh | Pulmonary Alveoli | es_ES |
dc.subject.mesh | Receptors, CCR5 | es_ES |
dc.subject.mesh | Viral Load | es_ES |
dc.subject.mesh | Virus Replication | es_ES |
dc.subject.mesh | Pandemics | es_ES |
dc.title | Characterization in vitro and in vivo of a pandemic H1N1 influenza virus from a fatal case | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Atribución 4.0 Internacional | * |
dc.identifier.pubmedID | 23326447 | es_ES |
dc.format.volume | 8 | es_ES |
dc.format.number | 1 | es_ES |
dc.format.page | e53515 | es_ES |
dc.identifier.doi | 10.1371/journal.pone.0053515 | es_ES |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 1932-6203 | es_ES |
dc.relation.publisherversion | https://doi.org/10.1371/journal.pone.0053515 | es_ES |
dc.identifier.journal | PloS one | es_ES |
dc.repisalud.centro | ISCIII::Centro Nacional de Microbiología | es_ES |
dc.repisalud.institucion | ISCIII | es_ES |
dc.rights.accessRights | open access | es_ES |