Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/6567
Título
CXCL6 is an important paracrine factor in the pro-angiogenic human cardiac progenitor-like cell secretome
Autor(es)
Toran, Jose Luis CNIC | Aguilar, Susana CNIC | Lopez, Juan Antonio CNIC | Torroja, Carlos CNIC | Quintana, Juan A. CNIC | Santiago, Cesar | Abad, José Luis | Gomes-Alves, Patricia | Gonzalez, Andres CNIC | Bernal, Juan Antonio CNIC | Jimenez-Borreguero, Luis J. CNIC | Alves, Paula Marques | R-Borlado, Luis | Vazquez, Jesus CNIC | Bernad, Antonio CNIC
Fecha de publicación
2017-10-02
Cita
Sci Rep. 2017; 7(1):12490
Idioma
Inglés
Tipo de documento
journal article
Resumen
Studies in recent years have established that the principal effects in cardiac cell therapy are associated with paracrine/autocrine factors. We combined several complementary techniques to define human cardiac progenitor cell (CPC) secretome constituted by 914 proteins/genes; 51% of these are associated with the exosomal compartment. To define the set of proteins specifically or highly differentially secreted by CPC, we compared human mesenchymal stem cells and dermal fibroblasts; the study defined a group of growth factors, cytokines and chemokines expressed at high to medium levels by CPC. Among them, IL-1, GROa (CXCL1), CXCL6 (GCP2) and IL-8 are examples whose expression was confirmed by most techniques used. ELISA showed that CXCL6 is significantly overexpressed in CPC conditioned medium (CM) (18- to 26-fold) and western blot confirmed expression of its receptors CXCR1 and CXCR2. Addition of anti-CXCL6 completely abolished migration in CPC-CM compared with anti-CXCR2, which promoted partial inhibition, and anti-CXCR1, which was inefficient. Anti-CXCL6 also significantly inhibited CPC CM angiogenic activity. In vivo evaluation also supported a relevant role for angiogenesis. Altogether, these results suggest a notable angiogenic potential in CPC-CM and identify CXCL6 as an important paracrine factor for CPC that signals mainly through CXCR2.
Palabras clave
Versión en línea
DOI
Aparece en las colecciones
Ficheros en el ítem
- Nombre:
- Copia de 41598_2017_11976_MOES ...
- Tamaño:
- 39.98Kb
- Formato:
- Microsoft Excel 2007
- Nombre:
- Copia de 41598_2017_11976_MOES ...
- Tamaño:
- 19.56Kb
- Formato:
- Microsoft Excel 2007
- Nombre:
- Copia de 41598_2017_11976_MOES ...
- Tamaño:
- 54.69Kb
- Formato:
- Microsoft Excel 2007
- Nombre:
- Copia de 41598_2017_11976_MOES ...
- Tamaño:
- 18.54Kb
- Formato:
- Microsoft Excel 2007