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dc.contributor.authorToran, Jose Luis 
dc.contributor.authorAguilar, Susana 
dc.contributor.authorLopez, Juan Antonio 
dc.contributor.authorTorroja, Carlos 
dc.contributor.authorQuintana, Juan A. 
dc.contributor.authorSantiago, Cesar
dc.contributor.authorAbad, José Luis
dc.contributor.authorGomes-Alves, Patricia
dc.contributor.authorGonzalez, Andres 
dc.contributor.authorBernal, Juan Antonio 
dc.contributor.authorJimenez-Borreguero, Luis J. 
dc.contributor.authorAlves, Paula Marques
dc.contributor.authorR-Borlado, Luis
dc.contributor.authorVazquez, Jesus 
dc.contributor.authorBernad, Antonio
dc.identifier.citationSci Rep. 2017; 7(1):12490es_ES
dc.description.abstractStudies in recent years have established that the principal effects in cardiac cell therapy are associated with paracrine/autocrine factors. We combined several complementary techniques to define human cardiac progenitor cell (CPC) secretome constituted by 914 proteins/genes; 51% of these are associated with the exosomal compartment. To define the set of proteins specifically or highly differentially secreted by CPC, we compared human mesenchymal stem cells and dermal fibroblasts; the study defined a group of growth factors, cytokines and chemokines expressed at high to medium levels by CPC. Among them, IL-1, GROa (CXCL1), CXCL6 (GCP2) and IL-8 are examples whose expression was confirmed by most techniques used. ELISA showed that CXCL6 is significantly overexpressed in CPC conditioned medium (CM) (18- to 26-fold) and western blot confirmed expression of its receptors CXCR1 and CXCR2. Addition of anti-CXCL6 completely abolished migration in CPC-CM compared with anti-CXCR2, which promoted partial inhibition, and anti-CXCR1, which was inefficient. Anti-CXCL6 also significantly inhibited CPC CM angiogenic activity. In vivo evaluation also supported a relevant role for angiogenesis. Altogether, these results suggest a notable angiogenic potential in CPC-CM and identify CXCL6 as an important paracrine factor for CPC that signals mainly through CXCR2.es_ES
dc.description.sponsorshipThis study was supported by funding from the European Commission (HEALTH-2009_242038) and by grants from the Spanish Ministry of Science and Innovation (SAF2012-34327 and SAF2015-70882-R to AB and BIO2012-37926 and BIO2015-67580-P to JV), the Research Program of the Comunidad Autónoma de Madrid (S2010/BMD-2420) and the Instituto de Salud Carlos III (RETICS-RD12/0019/0018 to AB and RETICS-RD12/0042/0056 to JV).es_ES
dc.relation.isversionofPublisher's versiones_ES
dc.subjectcardiac progenitor cellses_ES
dc.titleCXCL6 is an important paracrine factor in the pro-angiogenic human cardiac progenitor-like cell secretomees_ES
dc.rights.licenseAtribución-NoComercial-CompartirIgual 4.0 Internacional*
dc.contributor.funderEuropean Commisiones_ES
dc.contributor.funderMinisterio de Ciencia e Innovación (España)es_ES
dc.contributor.funderComunidad de Madrides_ES
dc.contributor.funderInstituto de Salud Carlos III - ISCIIIes_ES
dc.identifier.journalScientific reportses_ES
dc.repisalud.orgCNICCNIC::Unidades técnicas::Proteómica / Metabolómicaes_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Proteómica cardiovasculares_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Cardiomiopatías de origen genéticoes_ES
dc.repisalud.orgCNICCNIC::Unidades técnicas::Bioinformáticaes_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Antiguos CNICes_ES

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Atribución-NoComercial-CompartirIgual 4.0 Internacional
This item is licensed under a: Atribución-NoComercial-CompartirIgual 4.0 Internacional