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dc.contributor.author | Toran, Jose Luis | |
dc.contributor.author | Aguilar, Susana | |
dc.contributor.author | Lopez, Juan Antonio | |
dc.contributor.author | Torroja, Carlos | |
dc.contributor.author | Quintana, Juan A. | |
dc.contributor.author | Santiago, Cesar | |
dc.contributor.author | Abad, José Luis | |
dc.contributor.author | Gomes-Alves, Patricia | |
dc.contributor.author | Gonzalez, Andres | |
dc.contributor.author | Bernal, Juan Antonio | |
dc.contributor.author | Jimenez-Borreguero, Luis J. | |
dc.contributor.author | Alves, Paula Marques | |
dc.contributor.author | R-Borlado, Luis | |
dc.contributor.author | Vazquez, Jesus | |
dc.contributor.author | Bernad, Antonio | |
dc.date.accessioned | 2018-11-05T08:30:14Z | |
dc.date.available | 2018-11-05T08:30:14Z | |
dc.date.issued | 2017-10-02 | |
dc.identifier.citation | Sci Rep. 2017; 7(1):12490 | es_ES |
dc.identifier.issn | 2045-2322 | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/6567 | |
dc.description.abstract | Studies in recent years have established that the principal effects in cardiac cell therapy are associated with paracrine/autocrine factors. We combined several complementary techniques to define human cardiac progenitor cell (CPC) secretome constituted by 914 proteins/genes; 51% of these are associated with the exosomal compartment. To define the set of proteins specifically or highly differentially secreted by CPC, we compared human mesenchymal stem cells and dermal fibroblasts; the study defined a group of growth factors, cytokines and chemokines expressed at high to medium levels by CPC. Among them, IL-1, GROa (CXCL1), CXCL6 (GCP2) and IL-8 are examples whose expression was confirmed by most techniques used. ELISA showed that CXCL6 is significantly overexpressed in CPC conditioned medium (CM) (18- to 26-fold) and western blot confirmed expression of its receptors CXCR1 and CXCR2. Addition of anti-CXCL6 completely abolished migration in CPC-CM compared with anti-CXCR2, which promoted partial inhibition, and anti-CXCR1, which was inefficient. Anti-CXCL6 also significantly inhibited CPC CM angiogenic activity. In vivo evaluation also supported a relevant role for angiogenesis. Altogether, these results suggest a notable angiogenic potential in CPC-CM and identify CXCL6 as an important paracrine factor for CPC that signals mainly through CXCR2. | es_ES |
dc.description.sponsorship | This study was supported by funding from the European Commission (HEALTH-2009_242038) and by grants from the Spanish Ministry of Science and Innovation (SAF2012-34327 and SAF2015-70882-R to AB and BIO2012-37926 and BIO2015-67580-P to JV), the Research Program of the Comunidad Autónoma de Madrid (S2010/BMD-2420) and the Instituto de Salud Carlos III (RETICS-RD12/0019/0018 to AB and RETICS-RD12/0042/0056 to JV). | es_ES |
dc.language.iso | eng | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | * |
dc.subject | CXCL6 | es_ES |
dc.subject | Cardiac progenitor cells | es_ES |
dc.subject | Proteomics | es_ES |
dc.subject | Secretome | es_ES |
dc.title | CXCL6 is an important paracrine factor in the pro-angiogenic human cardiac progenitor-like cell secretome | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Atribución-NoComercial-CompartirIgual 4.0 Internacional | * |
dc.identifier.pubmedID | 28970523 | es_ES |
dc.format.volume | 7 | es_ES |
dc.format.number | 1 | es_ES |
dc.format.page | 12490 | es_ES |
dc.identifier.doi | 10.1038/s41598-017-11976-6 | es_ES |
dc.contributor.funder | Unión Europea. Comisión Europea | |
dc.contributor.funder | Ministerio de Ciencia e Innovación (España) | |
dc.contributor.funder | Comunidad de Madrid (España) | |
dc.contributor.funder | Instituto de Salud Carlos III | |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 2045-2322 | es_ES |
dc.relation.publisherversion | https://doi.org/10.1038/s41598-017-11976-6 | es_ES |
dc.identifier.journal | Scientific reports | es_ES |
dc.repisalud.orgCNIC | CNIC::Unidades técnicas::Proteómica / Metabolómica | es_ES |
dc.repisalud.orgCNIC | CNIC::Grupos de investigación::Proteómica cardiovascular | es_ES |
dc.repisalud.orgCNIC | CNIC::Grupos de investigación::Cardiomiopatías de origen genético | es_ES |
dc.repisalud.orgCNIC | CNIC::Unidades técnicas::Bioinformática | es_ES |
dc.repisalud.orgCNIC | CNIC::Grupos de investigación::Antiguos CNIC | es_ES |
dc.repisalud.institucion | CNIC | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/FP7/242038/EU | es_ES |
dc.rights.accessRights | open access | es_ES |