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dc.contributor.authorSala-Vila, Aleix 
dc.contributor.authorNavarro-Lerida, Inmaculada 
dc.contributor.authorSanchez-Alvarez, Miguel 
dc.contributor.authorBosch, Marta
dc.contributor.authorCalvo, Carlos
dc.contributor.authorLopez, Juan Antonio 
dc.contributor.authorCalvo, Enrique 
dc.contributor.authorFerguson, Charles
dc.contributor.authorGiacomello, Marta
dc.contributor.authorSerafini, Annalisa
dc.contributor.authorScorrano, Luca
dc.contributor.authorEnriquez, Jose Antonio 
dc.contributor.authorBalsinde, Jesus
dc.contributor.authorParton, Robert G.
dc.contributor.authorVazquez, Jesus 
dc.contributor.authorPol, Albert
dc.contributor.authordel Pozo, Miguel Angel
dc.identifier.citationSci Rep. 2016; 6:27351
dc.description.abstractThe mitochondria-associated membrane (MAM) is a specialized subdomain of the endoplasmic reticulum (ER) which acts as an intracellular signaling hub. MAM dysfunction has been related to liver disease. We report a high-throughput mass spectrometry-based proteomics characterization of MAMs from mouse liver, which portrays them as an extremely complex compartment involved in different metabolic processes, including steroid metabolism. Interestingly, we identified caveolin-1 (CAV1) as an integral component of hepatic MAMs, which determine the relative cholesterol content of these ER subdomains. Finally, a detailed comparative proteomics analysis between MAMs from wild type and CAV1-deficient mice suggests that functional CAV1 contributes to the recruitment and regulation of intracellular steroid and lipoprotein metabolism-related processes accrued at MAMs. The potential impact of these novel aspects of CAV1 biology on global cell homeostasis and disease is discussed.
dc.description.sponsorshipGrant support: M.A.d.P. was supported by grants SAF2011-25047, SAF2014-51876-R, and CSD2009-0016 from Spanish Ministry of Economy and Competitiveness (MINECO), 674/C/2013 from Fundacio La Marato de TV3, and AICR 15-0404 from the Worldwide Cancer Research Foundation. A.P. was supported by BFU2011-23745 and CSD2009-00016 from MINECO, and La Marato de TV3. A. S.-V., who is currently holding a Miguel Servet I contract (Instituto de Salud Carlos III, Spain), was a post-doctoral fellow at CNIC from Jan 2011 to Jan 2012 thanks to a Sara Borrell contract (Instituto de Salud Carlos III, Spain). I. N.-L. was supported by a postdoctoral fellowship from the Asociacion Espanola Contra el Cancer (AECC). The CNIC is supported by MINECO and the Pro CNIC Foundation, and is a Severo Ochoa Center of Excellence (MINECO award SEV-2015-0505). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
dc.publisherNature Publishing Group 
dc.subjectER MEMBRANES
dc.titleInterplay between hepatic mitochondria-associated membranes, lipid metabolism and caveolin-1 in mice
dc.typejournal article
dc.rights.licenseAtribución 4.0 Internacional*
dc.contributor.funderMinisterio de Economía y Competitividad (España) 
dc.contributor.funderFundación La Marató TV3 
dc.contributor.funderWorldwide Cancer Research 
dc.contributor.funderAsociación Española Contra el Cáncer 
dc.contributor.funderFundación ProCNIC 
dc.contributor.funderInstitució Catalana de Recerca i Estudis Avançats 
dc.identifier.journalScientific Reports
dc.repisalud.orgCNICCNIC::Grupos de investigación::Señalización por Integrinas
dc.repisalud.orgCNICCNIC::Grupos de investigación::Genómica Funcional
dc.repisalud.orgCNICCNIC::Grupos de investigación::Proteómica cardiovascular
dc.repisalud.orgCNICCNIC::Unidades técnicas::Proteómica / Metabolómica
dc.rights.accessRightsopen accesses_ES

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