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dc.contributor.author | Sala-Vila, Aleix | |
dc.contributor.author | Navarro-Lerida, Inmaculada | |
dc.contributor.author | Sanchez-Alvarez, Miguel | |
dc.contributor.author | Bosch, Marta | |
dc.contributor.author | Calvo, Carlos | |
dc.contributor.author | Lopez, Juan Antonio | |
dc.contributor.author | Calvo, Enrique | |
dc.contributor.author | Ferguson, Charles | |
dc.contributor.author | Giacomello, Marta | |
dc.contributor.author | Serafini, Annalisa | |
dc.contributor.author | Scorrano, Luca | |
dc.contributor.author | Enriquez, Jose Antonio | |
dc.contributor.author | Balsinde, Jesus | |
dc.contributor.author | Parton, Robert G. | |
dc.contributor.author | Vazquez, Jesus | |
dc.contributor.author | Pol, Albert | |
dc.contributor.author | del Pozo, Miguel Angel | |
dc.date.accessioned | 2017-10-30T13:15:44Z | |
dc.date.available | 2017-10-30T13:15:44Z | |
dc.date.issued | 2016 | |
dc.identifier | ISI:000377128700001 | |
dc.identifier.citation | Sci Rep. 2016; 6:27351 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/5222 | |
dc.description.abstract | The mitochondria-associated membrane (MAM) is a specialized subdomain of the endoplasmic reticulum (ER) which acts as an intracellular signaling hub. MAM dysfunction has been related to liver disease. We report a high-throughput mass spectrometry-based proteomics characterization of MAMs from mouse liver, which portrays them as an extremely complex compartment involved in different metabolic processes, including steroid metabolism. Interestingly, we identified caveolin-1 (CAV1) as an integral component of hepatic MAMs, which determine the relative cholesterol content of these ER subdomains. Finally, a detailed comparative proteomics analysis between MAMs from wild type and CAV1-deficient mice suggests that functional CAV1 contributes to the recruitment and regulation of intracellular steroid and lipoprotein metabolism-related processes accrued at MAMs. The potential impact of these novel aspects of CAV1 biology on global cell homeostasis and disease is discussed. | |
dc.description.sponsorship | Grant support: M.A.d.P. was supported by grants SAF2011-25047, SAF2014-51876-R, and CSD2009-0016 from Spanish Ministry of Economy and Competitiveness (MINECO), 674/C/2013 from Fundacio La Marato de TV3, and AICR 15-0404 from the Worldwide Cancer Research Foundation. A.P. was supported by BFU2011-23745 and CSD2009-00016 from MINECO, and La Marato de TV3. A. S.-V., who is currently holding a Miguel Servet I contract (Instituto de Salud Carlos III, Spain), was a post-doctoral fellow at CNIC from Jan 2011 to Jan 2012 thanks to a Sara Borrell contract (Instituto de Salud Carlos III, Spain). I. N.-L. was supported by a postdoctoral fellowship from the Asociacion Espanola Contra el Cancer (AECC). The CNIC is supported by MINECO and the Pro CNIC Foundation, and is a Severo Ochoa Center of Excellence (MINECO award SEV-2015-0505). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | |
dc.language.iso | eng | |
dc.publisher | Nature Publishing Group | |
dc.type.hasVersion | VoR | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | ER MEMBRANES | |
dc.subject | ALZHEIMER-DISEASE | |
dc.subject | CELLS | |
dc.subject | CHOLESTEROL | |
dc.subject | DYSFUNCTION | |
dc.subject | PROTEIN-1 | |
dc.subject | DROPLETS | |
dc.title | Interplay between hepatic mitochondria-associated membranes, lipid metabolism and caveolin-1 in mice | |
dc.type | journal article | |
dc.rights.license | Atribución 4.0 Internacional | * |
dc.identifier.pubmedID | 27272971 | |
dc.format.volume | 6 | |
dc.identifier.doi | 10.1038/srep27351 | |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | |
dc.contributor.funder | Fundación La Marató TV3 | |
dc.contributor.funder | Worldwide Cancer Research | |
dc.contributor.funder | Asociación Española Contra el Cáncer | |
dc.contributor.funder | Fundación ProCNIC | |
dc.contributor.funder | Institució Catalana de Recerca i Estudis Avançats | |
dc.description.peerreviewed | Sí | |
dc.relation.publisherversion | https://doi.org/10.1038/srep27351 | |
dc.identifier.journal | Scientific Reports | |
dc.repisalud.orgCNIC | CNIC::Grupos de investigación::Señalización por Integrinas | |
dc.repisalud.orgCNIC | CNIC::Grupos de investigación::Genómica Funcional | |
dc.repisalud.orgCNIC | CNIC::Grupos de investigación::Proteómica cardiovascular | |
dc.repisalud.orgCNIC | CNIC::Unidades técnicas::Proteómica / Metabolómica | |
dc.repisalud.institucion | CNIC | |
dc.relation.projectID | MINECO/ICTI2013-2016/SAF2014-51876-R | es_ES |
dc.relation.projectID | MINECO/ICTI2013-2016/SEV-2015-0505 | es_ES |
dc.rights.accessRights | open access | es_ES |