Show simple item record

dc.contributor.authorDomingo, Cristina 
dc.contributor.authorAlves, María Joao
dc.contributor.authorDe Ory, Fernando 
dc.contributor.authorTeichmann, Anette
dc.contributor.authorSchmitz, Herbert
dc.contributor.authorMüller, Rolf
dc.contributor.authorNiedrig, Matthias
dc.date.accessioned2017-09-04T16:28:03Z
dc.date.available2017-09-04T16:28:03Z
dc.date.issued2015-04-01
dc.identifier.citationBMC Infect Dis. 2015; 15: 167
dc.identifier.urihttp://hdl.handle.net/20.500.12105/4810
dc.description.abstractBACKGROUND: Dengue is endemic to the tropics and subtropics, and the most frequent of arthropod-borne viral diseases. Reliable diagnosis of dengue infection is important not only in clinical care but also in disease surveillance, the control of outbreaks, and the development of new vaccines. The diagnosis of dengue infection is usually established by a variety of commercial or in-house serological protocols. The European Network for the Diagnostics of Imported Viral Diseases (ENIVD) recognized the need to survey the accuracy of dengue serological diagnostics in current use, and organized an external quality assurance (EQA) study of dengue serological practice in diagnostic laboratories. METHODS: A 15-sample panel, consisting of sera reactive against dengue plus specificity and negative controls, was sent to 48 laboratories for serological testing. The results returned by the participating laboratories were anonymized, scored, and subjected to comparison and statistical analysis. RESULTS: Ten laboratories rated all samples correctly with regard to IgM, and only three achieved the full score for IgG detection. The main handicaps in assay performance were suboptimal sensitivity of in-house IgM detection protocols by comparison with better-performing commercial ELISA tests, and the presence of IgG cross-reactivity with heterologous flaviviruses. Differences of detail in the methodology of dengue IgG antibody detection appear to underlie the disparities in accuracy observed between laboratories. CONCLUSION: This EQA study demonstrates that there is room for many laboratories to improve sensitivity in the detection of anti-dengue virus IgM antibodies, against the benchmark set by commercial antibody capture ELISA tests. The EQA shows also that cross-reactivity is a continuing issue, and IgG detection protocols must be optimized to increase their specificity.
dc.language.isoeng
dc.publisherBioMed Central
dc.relation.isversionofPublisher's version
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectDengue
dc.subjectSerology
dc.subjectExternal quality assessment
dc.subjectDengue IgM
dc.subjectDengue IgG
dc.titleInternational external quality control assessment for the serological diagnosis of dengue infections
dc.typeArtículo
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID25886961
dc.format.volume15
dc.format.number1
dc.format.page167
dc.identifier.doi10.1186/s12879-015-0877-0
dc.description.peerreviewed
dc.identifier.e-issn1471-2334
dc.relation.publisherversionhttps://doi.org/10.1186/s12879-015-0877-0
dc.identifier.journalBMC Infectious Diseases
dc.repisalud.centroISCIII::Centro Nacional de Microbiología
dc.repisalud.institucionISCIII
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


Files in this item

Acceso Abierto
Thumbnail

This item appears in the following Collection(s)

Show simple item record

Atribución 4.0 Internacional
This item is licensed under a: Atribución 4.0 Internacional