Mostrar el registro sencillo del ítem
dc.contributor.author | Lacoma, A | |
dc.contributor.author | Cano, V | |
dc.contributor.author | Moranta, D | |
dc.contributor.author | Regueiro, V | |
dc.contributor.author | Dominguez-Villanueva, D | |
dc.contributor.author | Laabei, M | |
dc.contributor.author | González-Nicolau, María Del Mar | |
dc.contributor.author | Ausina, V | |
dc.contributor.author | Prat, C | |
dc.contributor.author | Bengoechea, Jose Antonio | |
dc.date.accessioned | 2024-07-11T09:12:16Z | |
dc.date.available | 2024-07-11T09:12:16Z | |
dc.date.issued | 2017 | |
dc.identifier.citation | Lacoma A., Cano García Victoria, Moranta Mesquida David, Regueiro V., Dominguez-Villanueva D., Laabei M., et al. Investigating intracellular persistence of Staphylococcus aureus within a murine alveolar macrophage cell line. Virulence. 2017;8(8):1761-1775. Epub 2017 Sep 12. | en |
dc.identifier.issn | 2150-5594 | |
dc.identifier.other | http://hdl.handle.net/20.500.13003/10008 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/20483 | |
dc.description.abstract | Objective: Staphylococcus aureus is a particularly difficult pathogen to eradicate from the respiratory tract. Previous studies have highlighted the intracellular capacity of S. aureus in several phagocytic and non-phagocytic cells. The aim of this study was to define S. aureus interaction within a murine alveolar macrophage cell line. Methods: Cell line MH-S was infected with Newman strain. Molecular mechanisms involved in phagocytosis were explored. To assess whether S. aureus survives intracellularly quantitative (gentamicin protection assays and bacterial plating) and qualitative analysis (immunofluorescence microscopy) were performed. Bacterial colocalization with different markers of the endocytic pathway was examined to characterize its intracellular trafficking. Results: We found that S. aureus uptake requires host actin polymerization, microtubule assembly and activation of phosphatidylinositol 3-kinase signaling. Time course experiments showed that Newman strain was able to persist within macrophages at least until 28.5 h post infection. We observed that intracellular bacteria are located inside an acidic subcellular compartment, which co-localizes with the late endosome/lysosome markers Lamp-1, Rab7 and RILP. Colocalization counts with TMR-dextran might reflect a balance between bacterial killing and intracellular survival. Conclusions: This study indicates that S. aureus persists and replicates inside murine alveolar macrophages, representing a privileged niche that can potentially offer protection from antimicrobial activity and immunological host defense mechanisms. | en |
dc.description.sponsorship | This work has been funded by the project PI13/01418 which is part of Plan Nacional de I+D +I and co-funded by ISCIII- Subdireccion General de Evaluacion and Fondo Europeo de Desarrollo Regional (FEDER). This work also received a grant from the Spanish Society of Pneumology and Thoracic Surgery (SEPAR 054/2011) and Instituto de Salud Carlos III: CIBERES (Corporate Research Program on Host-Pathogen interactions).; D. Dominguez-Villanueva is funded by Plan Nacional de I+D +I and co-funded by ISCIII- Subdireccion General de Evaluacion and Fondo Europeo de Desarrollo Regional (FEDER). M. Laabei was supported by a joint ERS/SEPAR fellowship (LTRF 2015). A. Lacoma has been a recipient of a grant from Sociedad Espanola de Microbiologia Clinica y Enfermedades Infecciosas (SEIMC) and from CIBERES (Programa de perfeccionamiento y movilidad). | es_ES |
dc.language.iso | eng | en |
dc.publisher | Taylor & Francis | en |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Invasion | |
dc.subject | Macrophage | |
dc.subject | Persistence | |
dc.subject | Phagocytosis | |
dc.subject | Staphylococcus aureus | |
dc.subject.mesh | Phagocytosis | * |
dc.subject.mesh | Animals | * |
dc.subject.mesh | Macrophages, Alveolar | * |
dc.subject.mesh | Microbial Viability | * |
dc.subject.mesh | Cell Line | * |
dc.subject.mesh | Staphylococcal Infections | * |
dc.subject.mesh | Staphylococcus aureus | * |
dc.subject.mesh | Mice | * |
dc.title | Investigating intracellular persistence of Staphylococcus aureus within a murine alveolar macrophage cell line | en |
dc.type | research article | en |
dc.rights.license | Attribution-NonCommercial-NoDerivatives 4.0 International | * |
dc.identifier.pubmedID | 28762868 | es_ES |
dc.format.volume | 8 | es_ES |
dc.format.number | 8 | es_ES |
dc.format.page | 1761-1775 | es_ES |
dc.identifier.doi | 10.1080/21505594.2017.1361089 | |
dc.identifier.e-issn | 2150-5608 | es_ES |
dc.relation.publisherversion | https://dx.doi.org/10.1080/21505594.2017.1361089 | en |
dc.identifier.journal | Virulence | es_ES |
dc.rights.accessRights | open access | en |
dc.subject.decs | Viabilidad Microbiana | * |
dc.subject.decs | Animales | * |
dc.subject.decs | Macrófagos Alveolares | * |
dc.subject.decs | Staphylococcus aureus | * |
dc.subject.decs | Infecciones Estafilocócicas | * |
dc.subject.decs | Fagocitosis | * |
dc.subject.decs | Ratones | * |
dc.subject.decs | Línea Celular | * |
dc.identifier.scopus | 2-s2.0-85029420665 | |
dc.identifier.wos | 419183300036 | |
dc.identifier.pui | L618297968 |
Ficheros en el ítem
Ficheros | Tamaño | Formato | Ver |
---|---|---|---|
No hay ficheros asociados a este ítem. |