Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/20449
Título
Positively selected amino acid replacements within the RuBisCO enzyme of oak trees are associated with ecological adaptations
Autor(es)
Fecha de publicación
2017-08-31
Cita
Hermida-Carrera C, Fares MA, Fernandez A, Gil-Pelegrin E, Kapralov MV, Mir Torres A, et al. Positively selected amino acid replacements within the RuBisCO enzyme of oak trees are associated with ecological adaptations. PLoS One. 2017 Aug 31;12(8):e0183970.
Idioma
Inglés
Tipo de documento
research article
Resumen
Phylogenetic analysis by maximum likelihood (PAML) has become the standard approach to study positive selection at the molecular level, but other methods may provide complementary ways to identify amino acid replacements associated with particular conditions. Here, we compare results of the decision tree (DT) model method with ones of PAML using the key photosynthetic enzyme RuBisCO as a model system to study molecular adaptation to particular ecological conditions in oaks (Quercus). We sequenced the chloroplast rbcL gene encoding RuBisCO large subunit in 158 Quercus species, covering about a third of the global genus diversity. It has been hypothesized that RuBisCO has evolved differentially depending on the environmental conditions and leaf traits governing internal gas diffusion patterns. Here, we show, using PAML, that amino acid replacements at the residue positions 95, 145, 251, 262 and 328 of the RuBisCO large subunit have been the subject of positive selection along particular Quercus lineages associated with the leaf traits and climate characteristics. In parallel, the DT model identified amino acid replacements at sites 95, 219, 262 and 328 being associated with the leaf traits and climate characteristics, exhibiting partial overlap with the results obtained using PAML.
MESH
Chloroplasts | Evolution, Molecular | Models, Molecular | Decision Trees | Quercus | Adaptation, Physiological | Climate | Mutation | Photosynthesis | Phylogeny | Amino Acid Substitution | Gene Expression | Plant Leaves | Ribulose-Bisphosphate Carboxylase | Selection, Genetic | Likelihood Functions | Protein Structure, Secondary
DECS
Sustitución de Aminoácidos | Expresión Génica | Funciones de Verosimilitud | Estructura Secundaria de Proteína | Hojas de la Planta | Mutación | Árboles de Decisión | Filogenia | Clima | Modelos Moleculares | Quercus | Fotosíntesis | Adaptación Fisiológica | Evolución Molecular | Cloroplastos | Selección Genética | Ribulosa-Bifosfato Carboxilasa
Versión en línea
DOI
Aparece en las colecciones
Acceso a texto completo