dc.contributor.author | López-Causapé, Carla | |
dc.contributor.author | Sommer, Lea Mette | |
dc.contributor.author | Cabot, Gabriel | |
dc.contributor.author | Rubio, Rosa | |
dc.contributor.author | Ocampo-Sosa, Alain A | |
dc.contributor.author | Johansen, Helle Krogh | |
dc.contributor.author | Figuerola Mulet, Joan | |
dc.contributor.author | Canton, Rafael | |
dc.contributor.author | Kidd, Timothy J | |
dc.contributor.author | Molin, Soeren | |
dc.contributor.author | Oliver, Antonio | |
dc.date.accessioned | 2024-07-11T09:10:38Z | |
dc.date.available | 2024-07-11T09:10:38Z | |
dc.date.issued | 2017-07-17 | |
dc.identifier.citation | López-Causapé C, Sommer LM, Cabot G, Rubio R, Ocampo-Sosa AA, Krogh Johansen H, et al. Evolution of the Pseudomonas aeruginosa mutational resistome in an international Cystic Fibrosis clone. Sci Rep. 2017 Jul 17;7:5555. | en |
dc.identifier.issn | 2045-2322 | |
dc.identifier.other | http://hdl.handle.net/20.500.13003/9744 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/20443 | |
dc.description.abstract | Emergence of epidemic clones and antibiotic resistance development compromises the management of Pseudomonas aeruginosa cystic fibrosis (CF) chronic respiratory infections. Whole genome sequencing (WGS) was used to decipher the phylogeny, interpatient dissemination, WGS mutator genotypes (mutome) and resistome of a widespread clone (CC274), in isolates from two highly-distant countries, Australia and Spain, covering an 18-year period. The coexistence of two divergent CC274 clonal lineages was revealed, but without evident geographical barrier; phylogenetic reconstructions and mutational resistome demonstrated the interpatient transmission of mutators. The extraordinary capacity of P. aeruginosa to develop resistance was evidenced by the emergence of mutations in > 100 genes related to antibiotic resistance during the evolution of CC274, catalyzed by mutator phenotypes. While the presence of classical mutational resistance mechanisms was confirmed and correlated with resistance phenotypes, results also showed a major role of unexpected mutations. Among them, PBP3 mutations, shaping up beta-lactam resistance, were noteworthy. A high selective pressure for mexZ mutations was evidenced, but we showed for the first time that high-level aminoglycoside resistance in CF is likely driven by mutations in fusA1/fusA2, coding for elongation factor G. Altogether, our results provide valuable information for understanding the evolution of the mutational resistome of CF P. aeruginosa. | en |
dc.description.sponsorship | We gratefully acknowledge the participants, associated staff (Women's and Children's Hospital, Adelaide; Royal Children's Hospital, Brisbane; Royal Children's Hospital, Melbourne; Mater Misericordiae Hospital, Brisbane; Children's Hospital at Westmead, Sydney, John Hunter Adult Hospital, Newcastle), Prof Scott Bell (QIMR Berghofer), Prof Claire Wainwright (The University of Queensland) and Prof Keith Grimwood (Griffith University) for their assistance with providing access to the Australian isolates used in this study. The contribution to this study of the Cystic Fibrosis Units from Hospital Ramon y Cajal and Hospital Son Espases is also acknowledged. This work was supported by the Ministerio de Economia y Competitividad of Spain, Instituto de Salud Carlos III, and was cofinanced by the European Regional Development Fund (ERDF) project A way to achieve Europe through the Spanish Network for Research in Infectious Diseases (REIPI) (RD12/0015 and RD16/0016) and grants PI15/00088. C.L.-C. received a fellowship from the Spanish Society of Microbiology and Infectious Diseases (SEIMC) and from the REIPI. T.J.K is a National Health and Medical Research Council Early Career Fellowship (GNT1088448). | es_ES |
dc.language.iso | eng | en |
dc.publisher | Nature Publishing Group | en |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject.mesh | beta-Lactam Resistance | * |
dc.subject.mesh | Australia | * |
dc.subject.mesh | Spain | * |
dc.subject.mesh | Microbial Sensitivity Tests | * |
dc.subject.mesh | Humans | * |
dc.subject.mesh | Penicillin-Binding Proteins | * |
dc.subject.mesh | Drug Resistance, Bacterial | * |
dc.subject.mesh | Cystic Fibrosis | * |
dc.subject.mesh | Fluoroquinolones | * |
dc.subject.mesh | Mutation | * |
dc.subject.mesh | Phylogeny | * |
dc.subject.mesh | Pseudomonas aeruginosa | * |
dc.subject.mesh | Polymyxins | * |
dc.subject.mesh | Prevalence | * |
dc.title | Evolution of the Pseudomonas aeruginosa mutational resistome in an international Cystic Fibrosis clone | en |
dc.type | research article | en |
dc.rights.license | Attribution 4.0 International | * |
dc.identifier.pubmedID | 28717172 | es_ES |
dc.format.volume | 7 | es_ES |
dc.format.page | 5555 | es_ES |
dc.identifier.doi | 10.1038/s41598-017-05621-5 | |
dc.relation.publisherversion | https://dx.doi.org/10.1038/s41598-017-05621-5 | en |
dc.identifier.journal | Scientific Reports | es_ES |
dc.rights.accessRights | open access | en |
dc.subject.decs | Proteínas de Unión a las Penicilinas | * |
dc.subject.decs | Prevalencia | * |
dc.subject.decs | Fluoroquinolonas | * |
dc.subject.decs | Farmacorresistencia Bacteriana | * |
dc.subject.decs | Mutación | * |
dc.subject.decs | Filogenia | * |
dc.subject.decs | Fibrosis Quística | * |
dc.subject.decs | Pruebas de Sensibilidad Microbiana | * |
dc.subject.decs | Humanos | * |
dc.subject.decs | Australia | * |
dc.subject.decs | Polimixinas | * |
dc.subject.decs | Pseudomonas aeruginosa | * |
dc.subject.decs | Resistencia betalactámica | * |
dc.subject.decs | España | * |
dc.identifier.scopus | 2-s2.0-85025116852 | |
dc.identifier.wos | 405675400035 | |
dc.identifier.pui | L625883985 | |