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dc.contributor.author | Phinney, Donald G | |
dc.contributor.author | Di Giuseppe, Michelangelo | |
dc.contributor.author | Njah, Joel | |
dc.contributor.author | Sala, Ernest | |
dc.contributor.author | Shiva, Sruti | |
dc.contributor.author | St Croix, Claudette M | |
dc.contributor.author | Stolz, Donna B | |
dc.contributor.author | Watkins, Simon C | |
dc.contributor.author | Di, Y. Peter | |
dc.contributor.author | Leikauf, George D | |
dc.contributor.author | Kolls, Jay | |
dc.contributor.author | Riches, David WH | |
dc.contributor.author | Deiuliis, Giuseppe | |
dc.contributor.author | Kaminski, Naftali | |
dc.contributor.author | Boregowda, Siddaraju V | |
dc.contributor.author | McKenna, David H | |
dc.contributor.author | Ortiz, Luis A | |
dc.date.accessioned | 2024-07-04T12:56:28Z | |
dc.date.available | 2024-07-04T12:56:28Z | |
dc.date.issued | 2015-10 | |
dc.identifier.citation | Phinney DG, Di Giuseppe M, Njah J, Sala-Llinas E, Shiva S, St Croix CM, et al. Mesenchymal stem cells use extracellular vesicles to outsource mitophagy and shuttle microRNAs. Nat Commun. 2015 Oct;6:8472. | en |
dc.identifier.issn | 2041-1723 | |
dc.identifier.other | http://hdl.handle.net/20.500.13003/10690 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/20147 | |
dc.description.abstract | Mesenchymal stem cells (MSCs) and macrophages are fundamental components of the stem cell niche and function coordinately to regulate haematopoietic stem cell self-renewal and mobilization. Recent studies indicate that mitophagy and healthy mitochondrial function are critical to the survival of stem cells, but how these processes are regulated in MSCs is unknown. Here we show that MSCs manage intracellular oxidative stress by targeting depolarized mitochondria to the plasma membrane via arrestin domain-containing protein 1-mediated microvesicles. The vesicles are then engulfed and re-utilized via a process involving fusion by macrophages, resulting in enhanced bioenergetics. Furthermore, we show that MSCs simultaneously shed micro RNA-containing exosomes that inhibit macrophage activation by suppressing Toll-like receptor signalling, thereby de-sensitizing macrophages to the ingested mitochondria. Collectively, these studies mechanistically link mitophagy and MSC survival with macrophage function, thereby providing a physiologically relevant context for the innate immunomodulatory activity of MSCs. | en |
dc.description.sponsorship | We thank Mr Brian Brockway for technical assistance with histological analysis and Dr Clotilde Thery from the Pasteur Institute for her advice regarding exosome isolation. This work was funded by National Institutes of Health grants to L.A.O. (R01HL114795, R01HL110334) and D.G.P. (R24 OD018254). Clinical grade human MSCs were provided by the NHLBI-sponsored Production Assistance for Cellular Therapies Program at the University of Minnesota (Contract HHSN268201000008C). | es_ES |
dc.language.iso | eng | en |
dc.publisher | Nature Publishing Group | en |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject.mesh | Oxidative Stress | * |
dc.subject.mesh | Toll-Like Receptor 9 | * |
dc.subject.mesh | Blotting, Western | * |
dc.subject.mesh | Mitochondria | * |
dc.subject.mesh | Extracellular Vesicles | * |
dc.subject.mesh | Silicosis | * |
dc.subject.mesh | Flow Cytometry | * |
dc.subject.mesh | Humans | * |
dc.subject.mesh | Arrestins | * |
dc.subject.mesh | Microscopy, Electron | * |
dc.subject.mesh | Toll-Like Receptors | * |
dc.subject.mesh | Exosomes | * |
dc.subject.mesh | Macrophages | * |
dc.subject.mesh | MicroRNAs | * |
dc.subject.mesh | Myeloid Differentiation Factor 88 | * |
dc.subject.mesh | Toll-Like Receptor 4 | * |
dc.subject.mesh | Cell-Derived Microparticles | * |
dc.subject.mesh | Animals | * |
dc.subject.mesh | Signal Transduction | * |
dc.subject.mesh | Receptors, Immunologic | * |
dc.subject.mesh | Mice | * |
dc.title | Mesenchymal stem cells use extracellular vesicles to outsource mitophagy and shuttle microRNAs | en |
dc.type | research article | en |
dc.rights.license | Attribution 4.0 International | * |
dc.identifier.pubmedID | 26442449 | es_ES |
dc.format.volume | 6 | es_ES |
dc.format.page | 8472 | es_ES |
dc.identifier.doi | 10.1038/ncomms9472 | |
dc.relation.publisherversion | https://dx.doi.org/10.1038/ncomms9472 | en |
dc.identifier.journal | Nature Communications | es_ES |
dc.rights.accessRights | open access | en |
dc.subject.decs | Transducción de Señal | * |
dc.subject.decs | Animales | * |
dc.subject.decs | Macrófagos | * |
dc.subject.decs | Citometría de Flujo | * |
dc.subject.decs | Silicosis | * |
dc.subject.decs | Receptor Toll-Like 4 | * |
dc.subject.decs | Humanos | * |
dc.subject.decs | Receptores Toll-Like | * |
dc.subject.decs | Arrestinas | * |
dc.subject.decs | Microscopía Electrónica | * |
dc.subject.decs | Vesículas Extracelulares | * |
dc.subject.decs | Receptor Toll-Like 9 | * |
dc.subject.decs | Estrés Oxidativo | * |
dc.subject.decs | Receptores Inmunológicos | * |
dc.subject.decs | Micropartículas Derivadas de Células | * |
dc.subject.decs | Ratones | * |
dc.subject.decs | Exosomas | * |
dc.subject.decs | Factor 88 de Diferenciación Mieloide | * |
dc.subject.decs | Mitocondrias | * |
dc.subject.decs | Western Blotting | * |
dc.subject.decs | MicroARNs | * |
dc.identifier.scopus | 2-s2.0-84943795327 | |
dc.identifier.wos | 364926700001 | |
dc.identifier.pui | L606304741 |
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