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dc.contributor.author | de Dios Caballero, Juan | |
dc.contributor.author | del Campo, Rosa | |
dc.contributor.author | Tato, Marta | |
dc.contributor.author | Gomez Garcia de la Pedrosa, Elia | |
dc.contributor.author | Cobo, Marta | |
dc.contributor.author | López-Causapé, Carla | |
dc.contributor.author | Gomez-Mampaso, Enrique | |
dc.contributor.author | Oliver, Antonio | |
dc.contributor.author | Canton, Rafael | |
dc.contributor.author | Spanish Network Cystic Fibrosis Mi | |
dc.date.accessioned | 2024-07-03T11:09:41Z | |
dc.date.available | 2024-07-03T11:09:41Z | |
dc.date.issued | 2014-12-24 | |
dc.identifier.citation | De Dios Caballero J, Del Campo R, Tato M, Gomez G De La Pedrosa E, Cobo M, López-Causapé C, et al. Microbiological diagnostic procedures for respiratory cystic fibrosis samples in Spain: towards standard of care practices. BMC Microbiol. 2014 Dec 24;14:335. | en |
dc.identifier.issn | 1471-2180 | |
dc.identifier.other | http://hdl.handle.net/20.500.13003/11037 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/20027 | |
dc.description.abstract | Background: The microbiological procedures for cystic fibrosis (CF) samples of 17 participating Spanish centers were examined to verify their compliance with current international and national guidelines and to implement the best standards of care for microbiology practices. A 47-item questionnaire covering different CF microbiology aspects was sent to participant laboratories. Telephone interviews were performed when necessary. Data about samples processing for bacteria, mycobacteria and fungi were collected. Results: Gene sequencing (71%), MALDI-TOF (59%) or both (94%) were available for most laboratories. Susceptibility testing was performed by automated microdilution systems (94%) and manual diffusion methods (59%). However, a low use of selective media for Staphylococcus aureus (59%) and Burkholderia cepacia complex (71%), and of epidemiological typing methods (41%) was reported. Conclusions: Most Spanish laboratories are in agreement with consensus guidelines for the processing of CF respiratory samples, but need to improve in the use of specific selective media and typing methods for epidemiologic studies. | en |
dc.description.sponsorship | JdDC is supported by a Rio Hortega contract (ref. CM14/00059) founded by Ministerio de Economia y Competitividad. Instituto de Salud Carlos III of Spain. The authors declare that this work was supported by Instituto de Salud Carlos III of Spain (research grants PI12/00103, PI13/00205 and PI12/00734) and cofinanced by the European Development Regional Fund (A Way to Achieve Europe program; Spanish Network for Research in Infectious Diseases grant REIPI RD12/0015). There are no competing financial or non-financial interests in the manuscript. | es_ES |
dc.language.iso | eng | en |
dc.publisher | BioMed Central (BMC) | en |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Cystic fibrosis | |
dc.subject | Microbiology | |
dc.subject | Laboratory procedures | |
dc.subject | Respiratory samples | |
dc.subject | Consensus guidelines | |
dc.subject.mesh | Burkholderia Infections | * |
dc.subject.mesh | Sputum | * |
dc.subject.mesh | Microbiological Techniques | * |
dc.subject.mesh | Spain | * |
dc.subject.mesh | Humans | * |
dc.subject.mesh | Laboratories | * |
dc.subject.mesh | Cystic Fibrosis | * |
dc.subject.mesh | Standard of Care | * |
dc.subject.mesh | Burkholderia cepacia | * |
dc.subject.mesh | Respiratory Tract Infections | * |
dc.subject.mesh | Specimen Handling | * |
dc.subject.mesh | Staphylococcal Infections | * |
dc.subject.mesh | Staphylococcus aureus | * |
dc.title | Microbiological diagnostic procedures for respiratory cystic fibrosis samples in Spain: towards standard of care practices | en |
dc.type | research article | en |
dc.rights.license | Attribution 4.0 International | * |
dc.identifier.pubmedID | 25927861 | es_ES |
dc.format.volume | 14 | es_ES |
dc.format.page | 335 | es_ES |
dc.identifier.doi | 10.1186/s12866-014-0335-y | |
dc.relation.publisherversion | https://dx.doi.org/10.1186/s12866-014-0335-y | en |
dc.identifier.journal | BMC Microbiology | es_ES |
dc.rights.accessRights | open access | en |
dc.subject.decs | Staphylococcus aureus | * |
dc.subject.decs | Manejo de Especímenes | * |
dc.subject.decs | Infecciones Estafilocócicas | * |
dc.subject.decs | Burkholderia cepacia | * |
dc.subject.decs | Fibrosis Quística | * |
dc.subject.decs | Humanos | * |
dc.subject.decs | Laboratorios | * |
dc.subject.decs | Infecciones por Burkholderia | * |
dc.subject.decs | Técnicas Microbiológicas | * |
dc.subject.decs | Esputo | * |
dc.subject.decs | España | * |
dc.subject.decs | Nivel de Atención | * |
dc.subject.decs | Infecciones del Sistema Respiratorio | * |
dc.identifier.scopus | 2-s2.0-84923874273 | |
dc.identifier.wos | 348183300001 | |
dc.identifier.pui | L602614249 |
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