Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/19251
Título
Arterialization requires the timely suppression of cell growth.
Autor(es)
Fecha de publicación
2021-01
Cita
Nature. 2021 Jan; 589(7842):437-441.
Idioma
Inglés
Tipo de documento
journal article
Resumen
The formation of arteries is thought to occur by the induction of a highly conserved arterial genetic programme in a subset of vessels that will later experience an increase in oxygenated blood flow1,2. The initial steps of arterial specification require both the VEGF and Notch signalling pathways3-5. Here, we combine inducible genetic mosaics and transcriptomics to modulate and define the function of these signalling pathways in cell proliferation, arteriovenous differentiation and mobilization. We show that endothelial cells with high levels of VEGF or Notch signalling are intrinsically biased to mobilize and form arteries; however, they are not genetically pre-determined, and can also form veins. Mechanistically, we found that increased levels of VEGF and Notch signalling in pre-arterial capillaries suppresses MYC-dependent metabolic and cell-cycle activities, and promotes the incorporation of endothelial cells into arteries. Mosaic lineage-tracing studies showed that endothelial cells that lack the Notch-RBPJ transcriptional activator complex rarely form arteries; however, these cells regained the ability to form arteries when the function of MYC was suppressed. Thus, the development of arteries does not require the direct induction of a Notch-dependent arterial differentiation programme, but instead depends on the timely suppression of endothelial cell-cycle progression and metabolism, a process that precedes arterial mobilization and complete differentiation.
MESH
Cell Proliferation | Adaptor Proteins, Signal Transducing | Animals | Arteries | Calcium-Binding Proteins | Cell Differentiation | Cell Line | Endothelial Cells | Endothelium, Vascular | Extracellular Signal-Regulated MAP Kinases | Female | Humans | Immunoglobulin J Recombination Signal Sequence-Binding Protein | Male | Mice | Mosaicism | Mutation | Phenotype | Proto-Oncogene Proteins c-myc | Receptors, Notch | Signal Transduction | Time Factors | Transcription, Genetic | Vascular Endothelial Growth Factor A | Vascular Endothelial Growth Factor Receptor-2 | Veins
Versión en línea
DOI
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