Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/18311
Título
Rapid amplitude-modulation of a diarylethene photoswitch: en route to contrast-enhanced fluorescence imaging
Autor(es)
Fecha de publicación
2021-04-15
Idioma
Inglés
Tipo de documento
research article
Resumen
A water soluble diarylethene (DAE) derivative that displays exceptionally intense fluorescence from the colorless open form has been synthesized and characterized using UV/vis spectroscopy and fluorescence microscopy. We show that the bright emission from the open form can be rapidly switched using amplitude modulated red light, that is, by light at wavelengths longer than those absorbed by the fluorescent species. This is highly appealing in any context where undesired background fluorescence disturbs the measurement, e.g., the autofluorescence commonly observed in fluorescence microscopy. We show that this scheme is conveniently applicable using lock-in detection, and that robust amplitude modulation of the probe fluorescence is indeed possible also in cell studies using fluorescence microscopy.
Palabras clave
Fluorescence | Microscopy, fluorescence | Fluorescent dyes | UV | Fluorescencia | Microscopía fluorescente | Colorantes fluorescentes | Rayos ultravioleta
MESH
Microscopy, Fluorescence | Spectrometry, Fluorescence | Fluorescent Dyes | Solubility | Light | Ultraviolet Rays | Microscopy | Cell Culture Techniques
Versión en línea
DOI
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