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dc.contributor.author | Torres-Ruiz, Raul | |
dc.contributor.author | Grazioso, Tatiana P | |
dc.contributor.author | Brandt, Marta | |
dc.contributor.author | Martinez-Lage, Marta | |
dc.contributor.author | Rodriguez Perales, Sandra | |
dc.contributor.author | Djouder, Nabil | |
dc.date.accessioned | 2024-02-08T14:31:48Z | |
dc.date.available | 2024-02-08T14:31:48Z | |
dc.date.issued | 2021-09-17 | |
dc.identifier.citation | STAR Protoc . 2021;2(3):100631. | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/17562 | |
dc.description.abstract | Chromosomal instability (CIN), a type of genomic instability, favors changes in chromosome number and structure and it is associated with the progression and initiation of multiple diseases, including cancer. Therefore, CIN identification and analysis represents a useful tool for cancer diagnosis and treatment. Here, we report an optimized molecular cytogenetic protocol to detect CIN in formalin-fixed, paraffin-embedded mouse and human tissues, using fluorescent in situ hybridization to visualize and quantify chromosomal alterations such as amplifications, deletions, and translocations. For complete information on the generation and use of this protocol, please refer to Brandt et al. (2018). | es_ES |
dc.description.sponsorship | This work was funded by grants to N.D. from the State Research Agency (AEI, 10.13039/501100011033) from the Spanish Ministry of Science and Innovation (projects SAF2016-76598-R, SAF2017-92733-EXP, RTI2018-094834-B-I00, and RED2018-102723-T) , cofounded by European Regional Development Fund (ERDF) . S.R-P. is supported by grants from the Spanish National Research and Development Plan, Instituto de Salud Carlos III, and Spanish Ministry of Science and Innovation cofounded by ERDF (PI17/02303; DTS19/00111 and BIO2017-91272-EXP) and by the Asociacion Espanola Contra el Cancer (AECC) . R.T.-R. was supported by a postdoctoral fellowship from the AECC. This work was developed at the CNIO funded by the Health Institute Carlos III (ISCIII) and the Spanish Ministry of Science and Innovation. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject.mesh | Chromosomal Instability | es_ES |
dc.subject.mesh | Interphase | es_ES |
dc.subject.mesh | Animals | es_ES |
dc.subject.mesh | Chromosomes, Artificial, Bacterial | es_ES |
dc.subject.mesh | DNA | es_ES |
dc.subject.mesh | Electrophoresis, Agar Gel | es_ES |
dc.subject.mesh | In Situ Hybridization, Fluorescence | es_ES |
dc.subject.mesh | Mice | es_ES |
dc.subject.mesh | Paraffin Embedding | es_ES |
dc.title | Detection of chromosome instability by interphase FISH in mouse and human tissues. | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.identifier.pubmedID | 34258592 | es_ES |
dc.format.volume | 2 | es_ES |
dc.format.number | 3 | es_ES |
dc.format.page | 100631 | es_ES |
dc.identifier.doi | 10.1016/j.xpro.2021.100631 | es_ES |
dc.contributor.funder | Ministerio de Ciencia e Innovación (España) | es_ES |
dc.contributor.funder | Instituto de Salud Carlos III | es_ES |
dc.contributor.funder | Asociación Española Contra el Cáncer | es_ES |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 2666-1667 | es_ES |
dc.relation.publisherversion | https://doi.org/10.1016/j.xpro.2021.100631. | es_ES |
dc.identifier.journal | STAR protocols | es_ES |
dc.repisalud.institucion | CNIO | es_ES |
dc.repisalud.orgCNIO | CNIO::Grupos de investigación::Grupo de Factores de Crecimiento, Nutrientes y Cáncer | es_ES |
dc.rights.accessRights | open access | es_ES |
dc.relation.projectFIS | info:eu-repo/grantAgreement/ES/SAF2016-76598-R | es_ES |
dc.relation.projectFIS | info:eu-repo/grantAgreement/ES/SAF2017-92733-EXP | es_ES |
dc.relation.projectFIS | info:eu-repo/grantAgreement/ES/SAF2017-92733-EXP | es_ES |
dc.relation.projectFIS | info:eu-repo/grantAgreement/ES/RTI2018-094834-B-I0 | es_ES |