Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/17562
Título
Detection of chromosome instability by interphase FISH in mouse and human tissues.
Autor(es)
Torres-Ruiz, Raul CNIC | Grazioso, Tatiana P | Brandt, Marta | Martinez-Lage, Marta | Rodriguez Perales, Sandra CNIO | Djouder, Nabil CNIO
Fecha de publicación
2021-09-17
Cita
STAR Protoc . 2021;2(3):100631.
Idioma
Inglés
Tipo de documento
journal article
Resumen
Chromosomal instability (CIN), a type of genomic instability, favors changes in chromosome number and structure and it is associated with the progression and initiation of multiple diseases, including cancer. Therefore, CIN identification and analysis represents a useful tool for cancer diagnosis and treatment. Here, we report an optimized molecular cytogenetic protocol to detect CIN in formalin-fixed, paraffin-embedded mouse and human tissues, using fluorescent in situ hybridization to visualize and quantify chromosomal alterations such as amplifications, deletions, and translocations. For complete information on the generation and use of this protocol, please refer to Brandt et al. (2018).
MESH
Chromosomal Instability | Interphase | Animals | Chromosomes, Artificial, Bacterial | DNA | Electrophoresis, Agar Gel | In Situ Hybridization, Fluorescence | Mice | Paraffin Embedding
Versión en línea
DOI
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