Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/17087
Título
Systemic administration of oleoylethanolamide protects from neuroinflammation and anhedonia induced by LPS in rats.
Autor(es)
Fecha de publicación
2015-04
Idioma
Inglés
Tipo de documento
research article
Resumen
BACKGROUND: The acylethanolamides oleoylethanolamide and palmitoylethanolamide are endogenous lipid mediators with proposed neuroprotectant properties in central nervous system (CNS) pathologies. The precise mechanisms remain partly unknown, but growing evidence suggests an antiinflammatory/antioxidant profile. METHODS: We tested whether oleoylethanolamide/palmitoylethanolamide (10 mg/kg, i.p.) attenuate neuroinflammation and acute phase responses (hypothalamus-pituitary-adrenal (HPA) stress axis stress axis activation, thermoregulation, and anhedonia) induced by lipopolysaccharide (0.5 mg/kg, i.p.) in rats. RESULTS: Lipopolysaccharide increased mRNA levels of the proinflammatory cytokines tumor necrosis factor-α, interleukin-1β, and interleukin-6, nuclear transcription factor-κB activity, and the expression of its inhibitory protein IκBα in cytoplasm, the inducible isoforms of nitric oxide synthase and cyclooxygenase-2, microsomal prostaglandin E2 synthase mRNA, and proinflammatory prostaglandin E2 content in frontal cortex 150 minutes after administration. As a result, the markers of nitrosative/oxidative stress nitrites (NO2(-)) and malondialdehyde were increased. Pretreatment with oleoylethanolamide/ palmitoylethanolamide reduced plasma tumor necrosis factor-α levels after lipopolysaccharide, but only oleoylethanolamide significantly reduced brain tumor necrosis factor-α mRNA. Oleoylethanolamide and palmitoylethanolamide prevented lipopolysaccharide-induced nuclear transcription factor-κB (NF-κB)/IκBα upregulation in nuclear and cytosolic extracts, respectively, the expression of inducible isoforms of nitric oxide synthase, cyclooxygenase-2, and microsomal prostaglandin E2 synthase and the levels of prostaglandin E2. Additionally, both acylethanolamides reduced lipopolysaccharide-induced oxidative/nitrosative stress. Neither oleoylethanolamide nor palmitoylethanolamide modified plasma corticosterone levels after lipopolysaccharide, but both acylethanolamides reduced the expression of hypothalamic markers of thermoregulation interleukin-1β, cyclooxygenase-2, and prostaglandin E2, and potentiated the hypothermic response after lipopolysaccharide. Interestingly, only oleoylethanolamide disrupted lipopolysaccharide-induced anhedonia in a saccharine preference test. CONCLUSIONS: Results indicate that oleoylethanolamide and palmitoylethanolamide have antiinflammatory/neuroprotective properties and suggest a role for these acylethanolamides as modulators of CNS pathologies with a neuroinflammatory component.
Palabras clave
OLEA | Lipopolysaccharide | Neuroinflammation | Anhedonia | Antiinflamatorios | Corticosterona | Citocinas | Modelos de enfermedad en animales | Encefalitis | Endocannabinoides | Endotoxinas | Etanolaminas | PEA | Preferencias alimenticias | Lóbulo frontal | Sistema hipotálamo-hipofisario | Mediadores de la inflamación | Peroxidación de lípidos | Fármacos neuroprotectores | Ácidos oleicos | Estrés oxidativo | Ácidos palmíticos | Sistema hipófiso-suprarrenal | Ratas wistar | Percepción del gusto
MESH
Animals | Anti-Inflammatory Agents | Behavior, Animal | Body Temperature Regulation | Brain | Corticosterone | Cytokines | Disease Models, Animal | Encephalitis | Endocannabinoids | Endotoxins | Ethanolamines | Food Preferences | Frontal Lobe | Hypothalamo-Hypophyseal System | Inflammation Mediators | Lipid Peroxidation | Male | Neuroprotective Agents | Oleic Acids | Oxidative Stress | Palmitic Acids | Pituitary-Adrenal System | Rats, Wistar | Taste Perception | Anhedonia
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