dc.contributor.author | Queipo-Ortuño, María Isabel | |
dc.contributor.author | Escoté, Xavier | |
dc.contributor.author | Ceperuelo-Mallafré, María Victoria | |
dc.contributor.author | Garrido-Sánchez, Lourdes | |
dc.contributor.author | Miranda, Merce | |
dc.contributor.author | Clemente-Postigo, Mercedes | |
dc.contributor.author | Pérez-Pérez, Rafael | |
dc.contributor.author | Peral, Belén | |
dc.contributor.author | Cardona Díaz, Fernando | |
dc.contributor.author | Fernández-Real, José Manuel | |
dc.contributor.author | Tinahones Madueño, Francisco José | |
dc.contributor.author | Vendrell, Joan | |
dc.date.accessioned | 2024-01-15T18:16:52Z | |
dc.date.available | 2024-01-15T18:16:52Z | |
dc.date.issued | 2012-11-05 | |
dc.identifier.other | http://hdl.handle.net/10668/845 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/17042 | |
dc.description.abstract | BACKGROUND: FABP4 is predominantly expressed in adipose tissue, and its circulating levels are linked with obesity and a poor atherogenic profile. OBJECTIVE: In patients with a wide BMI range, we analyze FABP4 expression in adipose and hepatic tissues in the settings of obesity and insulin resistance. Associations between FABP4 expression in adipose tissue and the FABP4 plasma level as well as the main adipogenic and lipolytic genes expressed in adipose tissue were also analyzed. METHODS: The expression of several lipogenic, lipolytic, PPAR family and FABP family genes was analyzed by real time PCR. FABP4 protein expression in total adipose tissues and its fractions were determined by western blot. RESULTS: In obesity FABP4 expression was down-regulated (at both mRNA and protein levels), with its levels mainly predicted by ATGL and inversely by the HOMA-IR index. The BMI appeared as the only determinant of the FABP4 variation in both adipose tissue depots. FABP4 plasma levels showed a significant progressive increase according to BMI but no association was detected between FABP4 circulating levels and SAT or VAT FABP4 gene expression. The gene expression of FABP1, FABP4 and FABP5 in hepatic tissue was significantly higher in tissue from the obese IR patients compared to the non-IR group. CONCLUSION: The inverse pattern in FABP4 expression between adipose and hepatic tissue observed in morbid obese patients, regarding the IR context, suggests that both tissues may act in a balanced manner. These differences may help us to understand the discrepancies between circulating plasma levels and adipose tissue expression in obesity. | |
dc.description.sponsorship | This work was supported by Fondo de Investigacion Sanitaria (FIS) 07/1024, 08/1195, CB06/03/0018, CP07/0095 and PI081655, 10/00967, 11/00085 from the Spanish Instituto de Salud Carlos III (ISCIII), Ministerio de Sanidad y Consumo; with the participation of the European Regional Development Fund (ERDF) SAF-2009-10461 from the Ministerio de Economia y Competitividad (MICINN), the Servicio Andaluz de Salud (PI325/2008) and Fundación Mutua Madrileñaa, Spain (BP). LGS and VCM are supported by fellowships from the Juan de la Cierva programme (JdlC) (JCI200904086 and JCI-2010-06395). CIBERs are an ISCIII Project. | |
dc.language.iso | eng | |
dc.publisher | Public Library of Science (PLOS) | |
dc.type.hasVersion | VoR | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Resistencia a la Insulina | |
dc.subject | Obesidad Mórbida | |
dc.subject | Obesidad | |
dc.subject | Proteínas de Unión a Ácidos Grasos | |
dc.subject | ARN Mensajero | |
dc.subject | Receptores Activados del Proliferador del Peroxisoma | |
dc.subject | Tejido Adiposo | |
dc.subject | Hígado | |
dc.subject | Experimentación Animal | |
dc.subject | Índice de Masa Corporal | |
dc.subject | Grasa Intraabdominal | |
dc.subject | Secuencia de Bases | |
dc.subject | Reacción en Cadena en Tiempo Real de la Polimerasa | |
dc.subject | Tejido Subcutáneo | |
dc.subject.mesh | Insulin Resistance | |
dc.subject.mesh | Obesity | |
dc.subject.mesh | Fatty Acid-Binding Proteins | |
dc.subject.mesh | RNA, Messenger | |
dc.subject.mesh | Peroxisome Proliferator-Activated Receptors | |
dc.subject.mesh | Adipose Tissue | |
dc.subject.mesh | Liver | |
dc.subject.mesh | Animal Experimentation | |
dc.subject.mesh | Body Mass Index | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Intra-Abdominal Fat | |
dc.subject.mesh | Base Sequence | |
dc.subject.mesh | Real-Time Polymerase Chain Reaction | |
dc.subject.mesh | Subcutaneous Tissue | |
dc.title | FABP4 Dynamics in Obesity: Discrepancies in Adipose Tissue and Liver Expression Regarding Circulating Plasma Levels. | |
dc.type | research article | |
dc.rights.license | Attribution 4.0 International | * |
dc.identifier.pubmedID | 23139800 | es_ES |
dc.identifier.doi | 10.1371/journal.pone.0048605 | |
dc.identifier.e-issn | 1932-6203 | es_ES |
dc.relation.publisherversion | http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0048605 | es |
dc.identifier.journal | PloS one | es_ES |
dc.rights.accessRights | open access | es_ES |
dc.contributor.authoraffiliation | [Queipo-Ortuño,MI; Ceperuelo-Mallafré,MV; Clemente-Postigo,M; Cardona Díaz,F] Laboratorio de Investigaciones Biomédicas del Hospital Universitario Virgen de la Victoria, Málaga, Spain. [Queipo-Ortuño,MI; Ceperuelo-Mallafré,MV; Clemente-Postigo,M; Cardona Díaz,F] Fundación IMABIS, Málaga, Spain. [Queipo-Ortuño,MI; Pérez-Pérez,R; Peral,B; Cardona Díaz,F; Fernández-Real,JM; Tinahones Madueño,FJ] CIBER de Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain. [Escoté,X; Garrido-Sánchez,L; Miranda,M; Vendrell,J] Endocrinology and Diabetes Unit.Joan XXIII University Hospital, IISPV, Universitat Rovira i Virgili, Tarragona, Spain. [Escoté,X; Ceperuelo-Mallafré,MV; Garrido-Sánchez,L; Miranda,M; Vendrell,J] CIBER de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Instituto de Salud Carlos III, Madrid, Spain. [Pérez-Pérez,R; Peral,B] Instituto de Investigaciones Biomédicas, Alberto Sols, Consejo Superior de Investigaciones Científicas (CSIC) & Universidad Autónoma de Madrid (UAM), Madrid, Spain. [Fernández-Real,JM] Diabetes, Endocrinology and Nutrition Service, Institut d’Investigació Biomèdica de Girona (IdIBGi), Girona, Spain. [Tinahones Madueño,FJ] Servicio Endocrinología y Nutrición del Hospital Universitario Virgen de la Victoria, Málaga, Spain. | |