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dc.contributor.author | Ballegeer, Marlies | |
dc.contributor.author | van Scherpenzeel, Revina C | |
dc.contributor.author | Delgado-Romero, Teresa | |
dc.contributor.author | Iglesias-Caballero, Maria | |
dc.contributor.author | Garcia-Barreno, Blanca | |
dc.contributor.author | Pandey, Shubham | |
dc.contributor.author | Rush, Scott A | |
dc.contributor.author | Kolkman, Joost A | |
dc.contributor.author | Mas-Lloret, Vicente | |
dc.contributor.author | McLellan, Jason S | |
dc.contributor.author | Saelens, Xavier | |
dc.date.accessioned | 2024-01-11T19:33:24Z | |
dc.date.available | 2024-01-11T19:33:24Z | |
dc.date.issued | 2024 | |
dc.identifier.citation | mBio. 2024 Jan 16;15(1):e0212223. | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/16942 | |
dc.description.abstract | Human metapneumovirus (hMPV) is an important respiratory pathogen for which no licensed antivirals or vaccines exist. Single-domain antibodies represent promising antiviral biologics that can be easily produced and formatted. We describe the isolation and detailed characterization of two hMPV-neutralizing single-domain antibodies that are directed against the fusion protein F. One of these single-domain antibodies broadly neutralizes hMPV A and B strains, can prevent proteolytic maturation of F, and binds to an epitope in the F trimer interface. This suggests that hMPV pre-F undergoes trimer opening or "breathing" on infectious virions, exposing a vulnerable site for neutralizing antibodies. Finally, we show that this single-domain antibody, fused to a human IgG1 Fc, can protect cotton rats against hMPV replication, an important finding for potential future clinical applications. | es_ES |
dc.description.sponsorship | This work was supported by a grant from Janssen Pharmaceuticals Inc. to X.S. and Welch Foundation grant number F-0003-19620604 to J.S.M. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | American Society for Microbiology (ASM) | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Fusion protein | es_ES |
dc.subject | Human metapneumovirus | es_ES |
dc.subject | Single-domain antibody | es_ES |
dc.subject | Structure | es_ES |
dc.title | A neutralizing single-domain antibody that targets the trimer interface of the human metapneumovirus fusion protein | es_ES |
dc.type | research article | es_ES |
dc.rights.license | Atribución 4.0 Internacional | * |
dc.identifier.pubmedID | 38117059 | es_ES |
dc.format.page | e0212223 | es_ES |
dc.identifier.doi | 10.1128/mbio.02122-23 | es_ES |
dc.contributor.funder | Welch Foundation | es_ES |
dc.contributor.funder | Janssen Cilag | es_ES |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 2150-7511 | es_ES |
dc.relation.publisherversion | https://doi.org/10.1128/mbio.02122-23 | es_ES |
dc.identifier.journal | mBio | es_ES |
dc.repisalud.centro | ISCIII::Centro Nacional de Microbiología | es_ES |
dc.repisalud.institucion | ISCIII | es_ES |
dc.rights.accessRights | open access | es_ES |