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dc.contributor.author | Vitallé, Joana | |
dc.contributor.author | Zenarruzabeitia, Olatz | |
dc.contributor.author | Merino-Pérez, Aitana | |
dc.contributor.author | Terrén, Iñigo | |
dc.contributor.author | Orrantia, Ane | |
dc.contributor.author | Pacho de Lucas, Arantza | |
dc.contributor.author | Iribarren, José A | |
dc.contributor.author | García-Fraile, Lucio J | |
dc.contributor.author | Balsalobre, Luz | |
dc.contributor.author | Amo, Laura | |
dc.contributor.author | Andres, Belen de | |
dc.contributor.author | Borrego, Francisco | |
dc.date.accessioned | 2023-12-18T19:41:41Z | |
dc.date.available | 2023-12-18T19:41:41Z | |
dc.date.issued | 2023-09-06 | |
dc.identifier.citation | Int J Mol Sci. 2023 Sep 6;24(18):13754. | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/16838 | |
dc.description.abstract | CD300a is differentially expressed among B cell subsets, although its expression in immunoglobulin (Ig)M+ B cells is not well known. We identified a B cell subset expressing CD300a and high levels of IgM (IgMhiCD300a+). The results showed that IgMhiCD300a+ B cells were CD10-CD27+CD25+IgDloCD21hiCD23-CD38loCD1chi, suggesting that they are circulating marginal zone (MZ) IgM memory B cells. Regarding the immunoglobulin repertoire, IgMhiCD300a+ B cells exhibited a higher mutation rate and usage of the IgH-VDJ genes than the IgM+CD300a- counterpart. Moreover, the shorter complementarity-determining region 3 (CDR3) amino acid (AA) length from IgMhiCD300a+ B cells together with the predicted antigen experience repertoire indicates that this B cell subset has a memory phenotype. IgM memory B cells are important in T cell-independent responses. Accordingly, we demonstrate that this particular subset secretes higher amounts of IgM after stimulation with pneumococcal polysaccharides or a toll-like receptor 9 (TLR9) agonist than IgM+CD300a- cells. Finally, the frequency of IgMhiCD300a+ B cells was lower in people living with HIV-1 (PLWH) and it was inversely correlated with the years with HIV infection. Altogether, these data help to identify a memory B cell subset that contributes to T cell-independent responses to pneumococcal infections and may explain the increase in severe pneumococcal infections and the impaired responses to pneumococcal vaccination in PLWH. | es_ES |
dc.description.sponsorship | This work was supported by the following grants to F.B.: Agencia Estatal de Investigación (PID2019-109583RB-I00/AEI/10.13039/501100011033) and Gilead Fellowship Program (GLD15/00303). A grant to B.d.A.: Agencia Estatal de Investigación-ISCIII (PI22CIII/00030). J.V. and I.T. are recipients of a predoctoral contract funded by the Department of Education, Basque Government (PRE_2018_2_0242 and PRE_2021_2_0215). A.O and I.T. are recipients of a fellowship from the Jesús de Gangoiti Barrera Foundation (FJGB18/002 and FJGB19/002). L.A. is an Ikerbasque Research Fellow, Ikerbasque, Basque Foundation for Science. F.B. is an Ikerbasque Research Professor, Ikerbasque, Basque Foundation for Science. We want to particularly acknowledge the patients in this study for their participation, to the HIV BioBank and the collaborating centres for the generous gifts of the clinical samples used in this study. The HIV BioBank is supported by Instituto de Salud Carlos III (PT20/00138) and Networking Research Center on Bioengineering, Biomaterials and Nanomedicine, CIBER-BBN (CB22/01/00041). CoRIS cohort is supported by CIBER—Consorcio Centro de Investigación Biomédica en Red—(CB21/13/00091), Instituto de Salud Carlos III, Ministerio de Ciencia e Innovación and Unión Europea—NextGenerationEU. This study would not have been possible without the collaboration of all patients, medical and nursing staff and data managers who have taken part in the Project. CoRIS cohort is supported by CIBER—Consorcio Centro de Investigación Biomédica en Red—(CB21/13/00091), Instituto de Salud Carlos III, Ministerio de Ciencia e Innovación and Unión Europea—NextGenerationEU. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Multidisciplinary Digital Publishing Institute (MDPI) | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | CD300 | es_ES |
dc.subject | CD300a | es_ES |
dc.subject | IgM | es_ES |
dc.subject | IgD | es_ES |
dc.subject | B cell | es_ES |
dc.subject | Memory | es_ES |
dc.subject | Marginal zone | es_ES |
dc.subject | Pneumococcus | es_ES |
dc.subject | Polysaccharides | es_ES |
dc.subject | HIV | es_ES |
dc.subject.mesh | HIV Infections | es_ES |
dc.subject.mesh | Pneumococcal Infections | es_ES |
dc.subject.mesh | Humans | es_ES |
dc.subject.mesh | Memory B Cells | es_ES |
dc.subject.mesh | Streptococcus pneumoniae | es_ES |
dc.subject.mesh | Adjuvants, Immunologic | es_ES |
dc.subject.mesh | Complementarity Determining Regions | es_ES |
dc.subject.mesh | Immunoglobulin M | es_ES |
dc.title | Human IgMhiCD300a+ B Cells Are Circulating Marginal Zone Memory B Cells That Respond to Pneumococcal Polysaccharides and Their Frequency Is Decreased in People Living with HIV | es_ES |
dc.type | research article | es_ES |
dc.rights.license | Atribución 4.0 Internacional | * |
dc.identifier.pubmedID | 37762055 | es_ES |
dc.format.volume | 24 | es_ES |
dc.format.number | 18 | es_ES |
dc.format.page | 13754 | es_ES |
dc.identifier.doi | 10.3390/ijms241813754 | es_ES |
dc.contributor.funder | Agencia Estatal de Investigación (España) | es_ES |
dc.contributor.funder | Gilead Sciences (Spain) | es_ES |
dc.contributor.funder | Basque Government (España) | es_ES |
dc.contributor.funder | Fundación Jesús de Gangoiti Barrera | es_ES |
dc.contributor.funder | Ikerbasque - Basque Foundation for Science | es_ES |
dc.contributor.funder | Instituto de Salud Carlos III | es_ES |
dc.contributor.funder | Centro de Investigación Biomédica en Red - CIBERBBN (Bioingeniería, Biomateriales y Nanomedicina) | es_ES |
dc.contributor.funder | Ministerio de Ciencia e Innovación (España) | es_ES |
dc.contributor.funder | Unión Europea. Comisión Europea. NextGenerationEU | es_ES |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 1422-0067 | es_ES |
dc.relation.publisherversion | https://doi.org/10.3390/ijms241813754 | es_ES |
dc.identifier.journal | International journal of molecular sciences | es_ES |
dc.repisalud.centro | ISCIII::Centro Nacional de Microbiología | es_ES |
dc.repisalud.institucion | ISCIII | es_ES |
dc.rights.accessRights | open access | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/PID2019-109583RB-I00 | es_ES |
dc.relation.projectFIS | info:fis/Instituto de Salud Carlos III///PI22-ISCIII Proyectos de I+D+I en salud (AES 2022).Intramurales (2022)/PI22CIII/00030 | es_ES |
dc.relation.projectFIS | info:eu-repo/grantAgreement/ES/PT20/00138 | es_ES |
dc.relation.projectFIS | info:eu-repo/grantAgreement/ES/CB22/01/00041 | es_ES |
dc.relation.projectFIS | info:eu-repo/grantAgreement/ES/CB21/13/00091 | es_ES |
dc.relation.projectFIS | info:eu-repo/grantAgreement/ES/CB21/13/00091 | es_ES |