dc.contributor.author | Garcia-Barbazan, Irene | |
dc.contributor.author | Torres-Cano, Alba | |
dc.contributor.author | Garcia-Rodas, Rocio | |
dc.contributor.author | Sachse, Martin | |
dc.contributor.author | Luque, Daniel | |
dc.contributor.author | Megías, Diego | |
dc.contributor.author | Zaragoza, Oscar | |
dc.date.accessioned | 2023-12-15T18:51:32Z | |
dc.date.available | 2023-12-15T18:51:32Z | |
dc.date.issued | 2024 | |
dc.identifier.citation | mBio. 2024 Jan 16;15(1):e0254923. | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/16821 | |
dc.description.abstract | Cryptococcus neoformans is an excellent model to investigate fungal pathogenesis. This yeast can produce "titan cells," which are cells of an abnormally larger size that contribute to the persistence of the yeast in the host. In this work, we have used a new approach to characterize them by identifying drugs that inhibit this process. We have used a repurposing off-patent drug library, combined with an automatic method to image and analyze fungal cell size. In this way, we have identified many compounds that inhibit this transition. Interestingly, several compounds were antioxidants, allowing us to confirm that endogenous ROS and mitochondrial changes are important for titan cell formation. This work provides new evidence of the mechanisms required for titanization. Furthermore, the future characterization of the inhibitory mechanisms of the identified compounds by the scientific community will contribute to better understand the role of titan cells in virulence. | es_ES |
dc.description.sponsorship | Oscar Zaragoza was funded by grant PID2020-114546RB by MCIN/AEI/10.13039/501100011033 and by grant SAF2017-86912-R from the Spanish Ministry for Science and Innovation. Irene García-Barbazán was funded by the Ministry for Science and Innovation (contract FPI PRE2018-083436). Alba Torres-Cano was funded by the Ministry for Science and Innovation (contract FPI PRE2021-099486). Rocío García-Rodas was funded by a “Juan de la Cierva” Contract from the Spanish Ministry for Economics, Industry and Competitivity (reference: IJCI-2015-25683) | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | American Society for Microbiology (ASM) | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Cryptococcus neoformans | es_ES |
dc.subject | Mitochondria | es_ES |
dc.subject | Off-patent drug repurposing | es_ES |
dc.subject | Reactive oxygen species | es_ES |
dc.subject | Titan-like cells | es_ES |
dc.title | Accumulation of endogenous free radicals is required to induce titan-like cell formation in Cryptococcus neoformans | es_ES |
dc.type | research article | es_ES |
dc.rights.license | Atribución 4.0 Internacional | * |
dc.identifier.pubmedID | 38078728 | es_ES |
dc.format.page | e0254923 | es_ES |
dc.identifier.doi | 10.1128/mbio.02549-23 | es_ES |
dc.contributor.funder | Agencia Estatal de Investigación (España) | es_ES |
dc.contributor.funder | Ministerio de Ciencia e Innovación (España) | es_ES |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 2150-7511 | es_ES |
dc.relation.publisherversion | https://doi.org/10.1128/mbio.02549-23 | es_ES |
dc.identifier.journal | mBio | es_ES |
dc.repisalud.centro | ISCIII::Centro Nacional de Microbiología::Unidades Comunes Científico-Técnicas (UCCT) | es_ES |
dc.repisalud.institucion | ISCIII | es_ES |
dc.rights.accessRights | open access | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/PID2020-114546RB | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/SAF2017-86912-R | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/PRE2018-083436 | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/PRE2021-099486 | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/IJCI-2015-25683 | es_ES |