Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/16682
Título
Angiogenesis inhibitor or aggressiveness marker? The function of endostatin in cancer through electrochemical biosensing
Autor(es)
Fecha de publicación
2023-09-14
Cita
Bioelectrochemistry. 2023 Sep 14:155:108571.
Idioma
Inglés
Tipo de documento
research article
Resumen
This work reports the first electrochemical bioplatform developed for the determination of human endostatin (HE), a biomarker with recognized antiangiogenic potential whose elevated circulating levels have also been associated with the development of aggressive cancers. The developed electroanalytical biotool combines the benefits of using magnetic microparticles for the implementation of sandwich immunoassays and amperometric transduction on disposable carbon electrodes. A limit of detection (LOD) of 34.1 pg mL-1 for HE standards and a selectivity suitable for its foray into the clinical oncology area, are demonstrated. The determination of HE in clinical samples such as lysates and secretomes of colorectal cancer (CRC) cells, plasma, and tissue samples from patients with CRC in different stages, has been faced with satisfactory results showing the ability for discriminating the metastatic capabilities of cells and for identifying and staging CRC patients. The developed bioplatform allows precise quantitative determinations, requiring minimal pre-treatments and sample amounts in only 75 min. In addition, due to the instrumentation and the type of substrates used in the detection step, the biotool is compatible with implementation in multiplexed and/or point-of-need devices, features in which this bioplatform is advantageous with respect to the enzyme linked immunosorbent assay (ELISA) or immunoblotting technologies.
Palabras clave
Angiogenesis | Colorectal cancer aggressiveness | Electrochemical bioplatform | Endostatin | Plasma | Tissue
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