dc.contributor.author | Cordoba, Raul | |
dc.contributor.author | Alvarez, Beatriz | |
dc.contributor.author | Masso, Pilar | |
dc.contributor.author | Gonzalez, Fernando Ataulfo | |
dc.contributor.author | Conejo, Luz | |
dc.contributor.author | Velasco, Diego | |
dc.contributor.author | Alonso, Juan-Manuel | |
dc.contributor.author | Villarrubia, Jesus | |
dc.contributor.author | Cava, Fernando | |
dc.contributor.author | Llamas, Pilar | |
dc.date.accessioned | 2023-11-03T14:53:00Z | |
dc.date.available | 2023-11-03T14:53:00Z | |
dc.date.issued | 2016-11 | |
dc.identifier.citation | Cytometry B Clin Cytom . 2016 Nov;90(6):543-545. | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/16630 | |
dc.description.abstract | Double-hit lymphoma (DHL) is a rare type of lymphoma with concurrent chromosomal translocations of C-MYC with BCL2 or BCL6, associated with unfavorable prognosis. We describe a case of DHL in a 79-year-old female patient previously diagnosed with diffuse large B-cell lymphoma (DLBCL) with an early relapse in the ascitic fluid. A seven-color multiparametric flow cytometry immunophenotyping study of the ascitic fluid was carried out, and revealed 99.78% of large in size and high cellular complexity B-cells positive for CD19, CD10 (64.27%), CD45 dim, CD22 dim, CD25 (60%), CD43 bright, CD38 bright, and IgM (18.53%); and negative for CD20, CD5, CD23, CD79b, CD103, CD200, CD11c, and FMC7, and 78.99% without light chain expression and 21% with Lambda chain restriction. Due to the expression of CD19 and CD10 with overexpression of BCL-2 protein and due to CD43 and CD38 positivity detected, those cells showed features between DLBCL and Burkitt lymphoma. Fluorescence in situ hybridization (FISH) confirmed both c-MYC/IGH and BCL2/IGH rearrangement. Our findings may help to identify cases requiring additional cytogenetic analysis. © 2015 International Clinical Cytometry Society. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Wiley | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject.mesh | Aged | es_ES |
dc.subject.mesh | Ascitic Fluid | es_ES |
dc.subject.mesh | Burkitt Lymphoma | es_ES |
dc.subject.mesh | Female | es_ES |
dc.subject.mesh | Flow Cytometry | es_ES |
dc.subject.mesh | Humans | es_ES |
dc.subject.mesh | Immunophenotyping | es_ES |
dc.subject.mesh | Lymphoma, Large B-Cell, Diffuse | es_ES |
dc.title | The utility of multiparametric seven-color flow cytometry in the detection of double hit lymphoma in ascitic fluid samples. | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.identifier.pubmedID | 25612555 | es_ES |
dc.format.volume | 90 | es_ES |
dc.format.number | 6 | es_ES |
dc.format.page | 543 | es_ES |
dc.identifier.doi | 10.1002/cyto.b.21227 | es_ES |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 1552-4957 | es_ES |
dc.relation.publisherversion | 10.1002/cyto.b.21227 | es_ES |
dc.identifier.journal | Cytometry. Part B, Clinical cytometry | es_ES |
dc.repisalud.orgCNIC | CNIC::Unidades técnicas::Citometría de Flujo | es_ES |
dc.repisalud.institucion | CNIC | es_ES |
dc.rights.accessRights | open access | es_ES |