Please use this identifier to cite or link to this item:http://hdl.handle.net/20.500.12105/16630
Title
The utility of multiparametric seven-color flow cytometry in the detection of double hit lymphoma in ascitic fluid samples.
Author(s)
Date issued
2016-11
Citation
Cytometry B Clin Cytom . 2016 Nov;90(6):543-545.
Language
Inglés
Document type
journal article
Abstract
Double-hit lymphoma (DHL) is a rare type of lymphoma with concurrent chromosomal translocations of C-MYC with BCL2 or BCL6, associated with unfavorable prognosis. We describe a case of DHL in a 79-year-old female patient previously diagnosed with diffuse large B-cell lymphoma (DLBCL) with an early relapse in the ascitic fluid. A seven-color multiparametric flow cytometry immunophenotyping study of the ascitic fluid was carried out, and revealed 99.78% of large in size and high cellular complexity B-cells positive for CD19, CD10 (64.27%), CD45 dim, CD22 dim, CD25 (60%), CD43 bright, CD38 bright, and IgM (18.53%); and negative for CD20, CD5, CD23, CD79b, CD103, CD200, CD11c, and FMC7, and 78.99% without light chain expression and 21% with Lambda chain restriction. Due to the expression of CD19 and CD10 with overexpression of BCL-2 protein and due to CD43 and CD38 positivity detected, those cells showed features between DLBCL and Burkitt lymphoma. Fluorescence in situ hybridization (FISH) confirmed both c-MYC/IGH and BCL2/IGH rearrangement. Our findings may help to identify cases requiring additional cytogenetic analysis. © 2015 International Clinical Cytometry Society.
MESH
Aged | Ascitic Fluid | Burkitt Lymphoma | Female | Flow Cytometry | Humans | Immunophenotyping | Lymphoma, Large B-Cell, Diffuse
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DOI
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