Show simple item record

dc.contributor.authorMacias, Alicia
dc.contributor.authorMartín, Paloma
dc.contributor.authorPerez-Olmeda, Mayte 
dc.contributor.authorFernandez Martinez, Beatriz 
dc.contributor.authorGomez-Barroso, Diana 
dc.contributor.authorFernández, Esperanza
dc.contributor.authorRamos, Julian Mauro
dc.contributor.authorHerrero-Romero, Laura 
dc.contributor.authorRodríguez, Saray
dc.contributor.authorDelgado, Elena 
dc.contributor.authorSánchez-Seco, María Paz 
dc.contributor.authorGalán, Miguel
dc.contributor.authorCorbacho, Antonio Jesús
dc.contributor.authorJimenez, Manuel
dc.contributor.authorMontero-Peña, Cristian
dc.contributor.authorValle, Antonio
dc.contributor.authorVazquez, Ana
dc.identifier.citationFront Cell Infect Microbiol. 2023 Jul 4;13:1155867.es_ES
dc.description.abstractIn Spain, the largest human West Nile virus (WNV) outbreak among humans was reported in 2020, constituting the second most important outbreak in Europe that season. Extremadura (southwestern Spain) was one of the affected areas, reporting six human cases. The first autochthonous human case in Spain was reported in Extremadura in 2004, and no other human cases were reported until 2020. In this work, we describe the first WNV human outbreak registered in Extremadura, focusing on the most important clinical aspects, diagnostic results, and control actions which followed. In 2020, from September to October, human WNV infections were diagnosed using a combination of molecular and serological methods (an in-house specific qRT-PCR and a commercial ELISA for anti-WNV IgM and IgG antibodies) and by analysing serum, urine, and/or cerebrospinal fluid samples. Serological positive serum samples were further tested using commercial kits against related flaviviruses Usutu and Tick-borne encephalitis in order to analyse serological reactivity and to confirm the results by neutralisation assays. In total, six cases of WNV infection (five with neuroinvasive disease and one with fever) were identified. Clinical presentation and laboratory findings are described. No viral RNA was detected in any of the analysed samples, but serological cross-reactivity was detected against the other tested flaviviruses. Molecular and serological methods for WNV detection in various samples as well as differential diagnosis are recommended. The largest number of human cases of WNV infection ever registered in Extremadura, Spain, occurred in 2020 in areas where circulation of WNV and other flaviviruses has been previously reported in humans and animals. Therefore, it is necessary to enhance surveillance not only for the early detection and implementation of response measures for WNV but also for other emerging flaviviruses that could be endemic in this area.es_ES
dc.description.sponsorshipThis research was partially funded by the project PI19CIII/00014 from the Instituto de Salud Carlos III.es_ES
dc.publisherFrontiers Media es_ES
dc.subjectWest Nile viruses_ES
dc.subjectHuman infectiones_ES
dc.subjectMolecular and serological methodses_ES
dc.subject.meshWest Nile virus es_ES
dc.subject.meshWest Nile Fever es_ES
dc.subject.meshFlavivirus es_ES
dc.subject.meshAnimals es_ES
dc.subject.meshHumans es_ES
dc.subject.meshSpain es_ES
dc.subject.meshAntibodies, Viral es_ES
dc.titleWest Nile virus emergence in humans in Extremadura, Spain 2020es_ES
dc.typeresearch articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.contributor.funderInstituto de Salud Carlos III es_ES
dc.identifier.journalFrontiers in cellular and infection microbiologyes_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.centroISCIII::Centro Nacional de Epidemiologíaes_ES
dc.rights.accessRightsopen accesses_ES
dc.relation.projectFISinfo:fis/Instituto de Salud Carlos III/Programa Estatal de Generación de Conocimiento y Fortalecimiento del Sistema Español de I+D+I/Subprograma Estatal de Generación de Conocimiento/PI19-ISCIII Modalidad Proyectos de Investigacion en Salud Intramurales. (2019)/PI19CIII/00014es_ES

Files in this item

Acceso Abierto

This item appears in the following Collection(s)

Show simple item record

Atribución 4.0 Internacional
This item is licensed under a: Atribución 4.0 Internacional