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dc.contributor.authorVidal-Alcántara, Erick Joan 
dc.contributor.authorMas-Lloret, Vicente 
dc.contributor.authorYélamos, María Belén
dc.contributor.authorGómez, Julián
dc.contributor.authorAmigot-Sánchez, Rafael 
dc.contributor.authorResino, Salvador 
dc.contributor.authorMartinez, Isidoro
dc.identifier.citationFront Mol Biosci. 2023 Jul 13;10:1225553.es_ES
dc.description.abstractBackground: Despite highly effective treatments to cure hepatitis C, almost 80% of chronically HCV-infected people are not treated, as they are unaware of their infection. Diagnostic rates and linkage to care must be substantially improved to reverse this situation. The HCV core antigen (HCVcAg) is a highly conserved protein that can be detected in the blood of HCV-infected patients and indicates active infection. Aim: To produce murine monoclonal antibodies against HCVcAg suitable for rapid and inexpensive tests to detect HCV infection. Methods: BALB/c mice were sequentially inoculated with purified recombinant HCVcAg from Gt1a, Gt3a, Gt4a, and Gt1b genotypes. Hybridomas producing the desired monoclonal antibodies were selected, and the reactivity of antibodies against HCVcAg from various genotypes was tested by Western blotting and dot blotting. The binding kinetics of the antibodies to purified HCVcAg was analyzed by surface plasmon resonance (SPR), and their ability to detect HCVcAg was tested by double antibody sandwich ELISA (DAS-ELISA). Results: Four specific monoclonal antibodies (1C, 2C, 4C, and 8C) were obtained. 1C, 2C, and 4C recognized HCVcAg of all genotypes tested (Gt1a, Gt1b, Gt2a, Gt3a, and Gt4a), while 8C did not recognize the Gt2a and Gt3a genotypes. Based on SPR data, the antibody-HCVcAg complexes formed are stable, with 2C having the strongest binding properties. DAS-ELISA with different antibody combinations easily detected HCVcAg in culture supernatants from HCV-infected cells. Conclusion: Specific and cross-reactive anti-HCVcAg monoclonal antibodies with strong binding properties were obtained that may be useful for detecting HCVcAg in HCV-infected samples.es_ES
dc.description.sponsorshipThis study was supported by a grant from Instituto de Salud Carlos III (ISCIII, PI19CIII/00009). The study was also funded by the Centro de Investigación Biomédica en Red de Enfermedades infecciosas (CIBERINFEC) [CB21/13/00044 (SR and IM)].es_ES
dc.publisherFrontiers Media es_ES
dc.subjectHepatitis Ces_ES
dc.subjectCore antigenes_ES
dc.subjectMonoclonal antibodyes_ES
dc.subjectRapid diagnostic testes_ES
dc.titleProduction and characterization of monoclonal antibodies for the detection of the hepatitis C core antigenes_ES
dc.typeresearch articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.contributor.funderInstituto de Salud Carlos III es_ES
dc.contributor.funderCentro de Investigación Biomédica en Red - CIBERINFEC (Enfermedades Infecciosas) es_ES
dc.identifier.journalFrontiers in molecular bioscienceses_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.rights.accessRightsopen accesses_ES
dc.relation.projectFISinfo:fis/Instituto de Salud Carlos III/Programa Estatal de Generación de Conocimiento y Fortalecimiento del Sistema Español de I+D+I/Subprograma Estatal de Generación de Conocimiento/PI19-ISCIII Modalidad Proyectos de Investigacion en Salud Intramurales. (2019)/PI19CIII/00009es_ES

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Atribución 4.0 Internacional
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