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dc.contributor.author | Lu, Stephen | |
dc.contributor.author | Martin-Martin, Ines | |
dc.contributor.author | Ribeiro, Jose M | |
dc.contributor.author | Calvo, Eric | |
dc.date.accessioned | 2023-07-13T10:46:17Z | |
dc.date.available | 2023-07-13T10:46:17Z | |
dc.date.issued | 2023-03-20 | |
dc.identifier.citation | BMC Genomics. 2023 Mar 20;24(1):135. | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/16237 | |
dc.description.abstract | Introduction: During evolution, blood-feeding arthropods developed a complex salivary mixture that can interfere with host haemostatic and immune response, favoring blood acquisition and pathogen transmission. Therefore, a survey of the salivary gland contents can lead to the identification of molecules with potent pharmacological activity in addition to increase our understanding of the molecular mechanisms underlying the hematophagic behaviour of arthropods. The southern house mosquito, Culex quinquefasciatus, is a vector of several pathogenic agents, including viruses and filarial parasites that can affect humans and wild animals. Results: Previously, a Sanger-based transcriptome of the salivary glands (sialome) of adult C. quinquefasciatus females was published based on the sequencing of 503 clones organized into 281 clusters. Here, we revisited the southern mosquito sialome using an Illumina-based RNA-sequencing approach of both male and female salivary glands. Our analysis resulted in the identification of 7,539 coding DNA sequences (CDS) that were functionally annotated into 25 classes, in addition to 159 long non-coding RNA (LncRNA). Additionally, comparison of male and female libraries allowed the identification of female-enriched transcripts that are potentially related to blood acquisition and/or pathogen transmission. Conclusion: Together, these findings represent an extended reference for the identification and characterization of the proteins containing relevant pharmacological activity in the salivary glands of C. quinquefasciatus mosquitoes. | es_ES |
dc.description.sponsorship | This work was supported by the Intramural Research Program of the Division of Intramural Research (AI001246 and AI000810), National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH). This work utilized the computational resources of the NIH HPC Biowulf cluster (http://hpc.nih.gov). Open Access funding provided by the National Institutes of Health (NIH). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | BioMed Central (BMC) | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Arthropod | es_ES |
dc.subject | Endonuclease | es_ES |
dc.subject | Evolution | es_ES |
dc.subject | Hematophagy | es_ES |
dc.subject | Medical entomolgy | es_ES |
dc.subject | Saliva | es_ES |
dc.subject.mesh | Culex | es_ES |
dc.subject.mesh | Culicidae | es_ES |
dc.subject.mesh | Humans | es_ES |
dc.subject.mesh | Animals | es_ES |
dc.subject.mesh | Male | es_ES |
dc.subject.mesh | Female | es_ES |
dc.subject.mesh | Mosquito Vectors | es_ES |
dc.subject.mesh | Proteins | es_ES |
dc.subject.mesh | Transcriptome | es_ES |
dc.title | A deeper insight into the sialome of male and female Culex quinquefasciatus mosquitoes | es_ES |
dc.type | research article | es_ES |
dc.rights.license | Atribución 4.0 Internacional | * |
dc.identifier.pubmedID | 36941562 | es_ES |
dc.format.volume | 24 | es_ES |
dc.format.number | 1 | es_ES |
dc.format.page | 135 | es_ES |
dc.identifier.doi | 10.1186/s12864-023-09236-1 | es_ES |
dc.contributor.funder | NIH - National Institute of Allergy and Infectious Diseases (NIAID) (Estados Unidos) | es_ES |
dc.contributor.funder | National Institutes of Health (Estados Unidos) | es_ES |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 1471-2164 | es_ES |
dc.relation.publisherversion | https://doi.org/10.1186/s12864-023-09236-1 | es_ES |
dc.identifier.journal | BMC genomics | es_ES |
dc.repisalud.centro | ISCIII::Centro Nacional de Microbiología | es_ES |
dc.repisalud.institucion | ISCIII | es_ES |
dc.rights.accessRights | open access | es_ES |