Please use this identifier to cite or link to this item:http://hdl.handle.net/20.500.12105/16052
PKD phosphorylation and COP9/Signalosome modulate intracellular Spry2 protein stability
Martinez, Natalia ISCIII | Gragera, Teresa ISCIII | de Lucas, Maria Pilar ISCIII | Camara, Ana Belen ISCIII | Ballester, Alicia ISCIII | Anta-Felez, Berta ISCIII | Fernández-Medarde, Alberto | López-Briones, Tania ISCIII | Ortega, Judith ISCIII | Peña-Jiménez, Daniel | Barbáchano, Antonio | Montero-Calle, Ana Maria ISCIII | Cordero, Víctor | Barderas Manchado, Rodrigo ISCIII | Iglesias, Teresa | Yunta, Mónica | Oliva-Martinez, Jose Luis ISCIII | Muñoz, Alberto | Santos, Eugenio | Zarich-Dimitrievich, Natasha ISCIII | Rojas-Cabañeros, Jose Maria ISCIII
Oncogenesis. 2023 Apr 12;12(1):20.
Spry2 is a molecular modulator of tyrosine kinase receptor signaling pathways that has cancer-type-specific effects. Mammalian Spry2 protein undergoes tyrosine and serine phosphorylation in response to growth factor stimulation. Spry2 expression is distinctly altered in various cancer types. Inhibition of the proteasome functionality results in reduced intracellular Spry2 degradation. Using in vitro and in vivo assays, we show that protein kinase D (PKD) phosphorylates Spry2 at serine 112 and interacts in vivo with the C-terminal half of this protein. Importantly, missense mutation of Ser112 decreases the rate of Spry2 intracellular protein degradation. Either knocking down the expression of all three mammalian PKD isoforms or blocking their kinase activity with a specific inhibitor contributes to the stabilization of Spry2 wild-type protein. Downregulation of CSN3, a component of the COP9/Signalosome that binds PKD, significantly increases the half-life of Spry2 wild-type protein but does not affect the stability of a Spry2 after mutating Ser112 to the non-phosphorylatable residue alanine. Our data demonstrate that both PKD and the COP9/Signalosome play a significant role in control of Spry2 intracellular stability and support the consideration of the PKD/COP9 complex as a potential therapeutic target in tumors where Spry2 expression is reduced.
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