Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/15732
Título
ICAP-1 loss impairs CD8+ thymocyte development and leads to reduced marginal zone B cells in mice.
Autor(es)
Sevilla-Movilla, Silvia | Fuentes, Patricia | Rodríguez-García, Yaiza | Arellano-Sánchez, Nohemi | Krenn, Peter W | de Val, Soledad Isern | Montero-Herradón, Sara | García-Ceca, Javier | Burdiel-Herencia, Valeria | Gardeta, Sofía R | Aguilera-Montilla, Noemí | Barrio-Alonso, Celia | Crainiciuc, Georgiana CNIC | Bouvard, Daniel | García-Pardo, Angeles | Zapata, Agustin G | Hidalgo, Andres CNIC | Fässler, Reinhard | Carrasco, Yolanda R | Toribio, Maria L | Teixidó, Joaquin
Fecha de publicación
2022-08
Cita
Eur J Immunol. 2022 Aug;52(8):1228-1242
Idioma
Inglés
Tipo de documento
journal article
Resumen
ICAP-1 regulates β1-integrin activation and cell adhesion. Here, we used ICAP-1-null mice to study ICAP-1 potential involvement during immune cell development and function. Integrin α4β1-dependent adhesion was comparable between ICAP-1-null and control thymocytes, but lack of ICAP-1 caused a defective single-positive (SP) CD8+ cell generation, thus, unveiling an ICAP-1 involvement in SP thymocyte development. ICAP-1 bears a nuclear localization signal and we found it displayed a strong nuclear distribution in thymocytes. Interestingly, there was a direct correlation between the lack of ICAP-1 and reduced levels in SP CD8+ thymocytes of Runx3, a transcription factor required for CD8+ thymocyte generation. In the spleen, ICAP-1 was found evenly distributed between cytoplasm and nuclear fractions, and ICAP-1-/- spleen T and B cells displayed upregulation of α4β1-mediated adhesion, indicating that ICAP-1 negatively controls their attachment. Furthermore, CD3+ - and CD19+ -selected spleen cells from ICAP-1-null mice showed reduced proliferation in response to T- and B-cell stimuli, respectively. Finally, loss of ICAP-1 caused a remarkable decrease in marginal zone B- cell frequencies and a moderate increase in follicular B cells. Together, these data unravel an ICAP-1 involvement in the generation of SP CD8+ thymocytes and in the control of marginal zone B-cell numbers.
MESH
Adaptor Proteins, Signal Transducing | B-Lymphocytes | CD8-Positive T-Lymphocytes | Lymphocyte Activation | Thymocytes | Animals | Cell Differentiation | Integrin beta1 | Mice | Mice, Knockout | Spleen | Thymus Gland
Versión en línea
DOI
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