Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/15492
Título
Exploring the antigenic relatedness of influenza virus haemagglutinins with strain-specific polyclonal antibodies
Autor(es)
Garcia-Barreno, Blanca ISCIII | Delgado-Romero, Teresa ISCIII | Benito, Sonia ISCIII | Casas Flecha, Inmaculada ISCIII | Pozo Sanchez, Francisco ISCIII | Melero, Jose Antonio ISCIII
Fecha de publicación
2014-10
Cita
J Gen Virol. 2014 Oct;95(Pt 10):2140-2145.
Idioma
Inglés
Tipo de documento
journal article
Resumen
Alternative methods to the standard haemagglutination inhibition (HI) and neutralization tests to probe the antigenic properties of the influenza virus haemagglutinin (HA) were developed in this study. Vaccinia virus recombinants expressing reference HAs were used to immunize rabbits from which polyclonal antibodies were obtained. These antibodies were subtype specific but showed limited intra-subtype strain specificity in ELISA. The discriminatory capacity of these antibodies was, however, markedly increased after adsorption to cells infected with heterologous influenza viruses, revealing antigenic differences that were otherwise undistinguishable by standard HI and neutralization tests. Furthermore, the unadsorbed antibodies could be used to select escape mutants of the reference strain, which after sequencing unveiled amino acid changes responsible of the noted antigenic differences. These procedures therefore provide alternative methods for the antigenic characterization of influenza HA and might be useful in studies of HA antigenic evolution.
MESH
Cross Reactions | Animals | Antibodies, Viral | Enzyme-Linked Immunosorbent Assay | Genetic Vectors | Hemagglutinin Glycoproteins, Influenza Virus | Humans | Immunization | Orthomyxoviridae | Rabbits | Vaccinia virus
Versión en línea
DOI
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