Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/15294
Título
Dynamics of HIV Reservoir and HIV-1 Viral Splicing in HCV-Exposed Individuals after Elimination with DAAs or Spontaneous Clearance
Autor(es)
Martinez-Roman, Paula ISCIII | Crespo-Bermejo, Celia ISCIII | Valle-Millares, Daniel ISCIII | Lara-Aguilar, Violeta ISCIII | Arca de Lafuente, Sonia ISCIII | Martín-Carbonero, Luz | Ryan, Pablo | de Los Santos, Ignacio | Lopez-Huertas, Maria Rosa ISCIII | Palladino, Claudia | Muñoz-Muñoz, María | Fernandez-Rodriguez, Amanda ISCIII | Coiras, Mayte ISCIII | Briz, Veronica ISCIII | COVIHEP network
Fecha de publicación
2022-06-21
Cita
J Clin Med. 2022 Jun 21;11(13):3579.
Idioma
Inglés
Tipo de documento
journal article
Resumen
Background: Although human immunodeficiency virus type 1 (HIV-1) reservoir size is very stable under antiretroviral therapy (ART), individuals exposed to the Hepatitis C virus (HCV) (chronically coinfected and spontaneous clarifiers) show an increase in HIV reservoir size and in spliced viral RNA, which could indicate that the viral protein regulator Tat is being more actively synthesized and, thus, could lead to a higher yield of new HIV. However, it is still unknown whether the effect of HCV elimination with direct-acting antivirals (DAAs) could modify the HIV reservoir and splicing. Methods: This longitudinal study (48 weeks' follow-up after sustained virological response) involves 22 HIV+-monoinfected individuals, 17 HIV+/HCV- spontaneous clarifiers, and 24 HIV+/HCV+ chronically infected subjects who eliminated HCV with DAAs (all of them aviremic, viral load < 50). Viral-spliced RNA transcripts and proviral DNA copies were quantified by qPCR. Paired samples were analyzed using a mixed generalized linear model. Results: A decrease in HIV proviral DNA was observed in HIV+/HCV- subjects, but no significant differences were found for the other study groups. An increased production of multiple spliced transcripts was found in HIV+ and HIV+/HCV+ individuals. Conclusions: We conclude that elimination of HCV by DAAs was unable to revert the consequences derived from chronic HCV infection for the reservoir size and viral splicing, which could indicate an increased risk of rapid HIV-reservoir reactivation. Moreover, spontaneous clarifiers showed a significant decrease in the HIV reservoir, likely due to an enhanced immune response in these individuals.
Palabras clave
DAAs | HIV reservoir | HIV/HCV | Coinfection | Dynamics | Spontaneous HCV clearance | Viral splicing
Versión en línea
DOI
Aparece en las colecciones
Ficheros en el ítem
![Acceso Abierto Acceso Abierto](/themes/Mirage2/images/openAccess.png)
- Nombre:
- DynamicsOfHIVReservoir_2022.pdf
- Tamaño:
- 738.8Kb
- Formato:
- Descripción:
- Artículo principal
![Acceso Abierto Acceso Abierto](/themes/Mirage2/images/openAccess.png)
- Nombre:
- DynamicsOfHIVReservoirSuppleme ...
- Tamaño:
- 221.2Kb
- Formato:
- Descripción:
- Supplementary Information
![Acceso Abierto Acceso Abierto](/themes/Mirage2/images/openAccess.png)
- Nombre:
- DynamicsOfHIVReservoirSuppleme ...
- Tamaño:
- 173.1Kb
- Formato:
- Descripción:
- Supplementary Information
![Acceso Abierto Acceso Abierto](/themes/Mirage2/images/openAccess.png)
- Nombre:
- DynamicsOfHIVReservoirSuppleme ...
- Tamaño:
- 174.7Kb
- Formato:
- Descripción:
- Supplementary Information
![Acceso Abierto Acceso Abierto](/themes/Mirage2/images/openAccess.png)
- Nombre:
- DynamicsOfHIVReservoirSuppleme ...
- Tamaño:
- 187.2Kb
- Formato:
- Descripción:
- Supplementary Information
![Acceso Abierto Acceso Abierto](/themes/Mirage2/images/openAccess.png)
- Nombre:
- DynamicsOfHIVReservoirSuppleme ...
- Tamaño:
- 217.1Kb
- Formato:
- Descripción:
- Supplementary Information