Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/15135
Título
Usefulness of a commercial LAMP assay for detection of malaria infection, including Plasmodium knowlesi cases, in returning travelers in Spain
Autor(es)
Martin-Ramirez, Alexandra ISCIII | Lanza-Suarez, Marta ISCIII | Hisam, Shamilah | Perez-Ayala, Ana | Rubio Muñoz, Jose Miguel ISCIII
Fecha de publicación
2022-04-25
Cita
BMC Res Notes. 2022 Apr 25;15(1):147.
Idioma
Inglés
Tipo de documento
journal article
Resumen
Objetive: Main malaria diagnosis is based on microscopic examination combined with rapid diagnostic tests. Both methods have low sensitivity and specificity. Loop-mediated isothermal amplification techniques have shown a sensitivity similar to PCR but with lower times of performance. This study aimed to assess a commercial LAMP for the diagnosis of malaria (Alethia® Malaria) against the Nested-Multiplex-Malaria PCR, including the analytical sensitivity and the operational characteristics. Results: One hundred five samples out of 114 rendered valid results, obtaining 85 positive samples and 18 negative samples with an agreement of 98% compared to the reference method with a sensitivity, specificity and kappa coefficient of 98.84%, 94.74% and 0.94 respectively, with only two discrepant samples. The turnaround time was estimated in 1 h and 30 min, with a cost of 32.67€ per determination. The results showed several advantages of the Alethia® Malaria, as it was easy to perform, minimal training requirement and 40 min run. Moreover, it includes an internal control to avoid false negatives. However, it also showed some limitations such as the need for a specific amplification and detection device, the detection of only Plasmodium spp. and a very high price.
Palabras clave
Plasmodium | Molecular diagnosis | Plasmodium knowlesi | LAMP | Illumigene® Malaria | Alethia® | Malaria | PCR
MESH
Malaria | Plasmodium knowlesi | Humans | Molecular Diagnostic Techniques | Multiplex Polymerase Chain Reaction | Nucleic Acid Amplification Techniques | Spain
Versión en línea
DOI
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