Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/14749
Título
Long-Term Preservation and Storage of Faecal Samples in Whatman® Cards for PCR Detection and Genotyping of Giardia duodenalis and Cryptosporidium hominis
Autor(es)
Köster, Pamela Carolina ISCIII | Bailo, Begoña | Dashti, Alejandro ISCIII | Hernández-Castro, Carolina ISCIII | Calero-Bernal, Rafael | Ponce-Gordo, Francisco | Gonzalez-Barrio, David ISCIII | Carmena, David ISCIII
Fecha de publicación
2021-05-12
Cita
Animals (Basel). 2021 May 12;11(5):1369.
Idioma
Inglés
Tipo de documento
journal article
Resumen
Preservation and conservation of biological specimens, including faecal samples, is a challenge in remote areas or poor-resource settings where the cold chain cannot be maintained. This study aims at evaluating the suitability of filter cards for long-term storage of faecal samples of animal and human origin positive to the diarrhoea-causing protozoan parasites, Giardia duodenalis and Cryptosporidium hominis. Three commercially available Whatman® Filter Cards were comparatively assessed: the FTA® Classic Card, the FTA® Elute Micro Card, and the 903 Protein Saver Card. Human faecal samples positive to G. duodenalis (n = 5) and C. hominis (n = 5) were used to impregnate the selected cards at given storage (1 month, 3 months, and 6 months) periods and temperature (-20 °C, 4 °C, and room temperature) conditions. Parasite DNA was detected by PCR-based methods. Sensitivity assays and quality control procedures to assess suitability for genotyping purposes were conducted. Overall, all three Whatman® cards were proven useful for the detection and molecular characterisation of G. duodenalis and C. hominis under the evaluated conditions. Whatman® cards represent a simple, safe, and cost-effective option for the transportation, preservation, and storage of faecal samples without the need of the cold chain.
Palabras clave
Cryptosporidium hominis | Giardia duodenalis | PCR | Filter card | Preservation | Storage | Transportation | Faeces
Versión en línea
DOI
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