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dc.contributor.authorGarcia-Rios, Estefani 
dc.contributor.authorLeivas, Alejandra
dc.contributor.authorMancebo, Francisco Jose 
dc.contributor.authorSánchez-Vega, Laura
dc.contributor.authorLanzarot, Diego
dc.contributor.authorAguado, José María
dc.contributor.authorMartinez-Lopez, Joaquin 
dc.contributor.authorPaciello, María Liz
dc.contributor.authorPerez-Romero, Pilar 
dc.date.accessioned2022-05-24T09:42:05Z
dc.date.available2022-05-24T09:42:05Z
dc.date.issued2022-03-09
dc.identifier.citationBiomedicines. 2022 Mar 9;10(3):630.es_ES
dc.identifier.issn2227-9059es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/14481
dc.description.abstractIn order to demonstrate the feasibility of preparing clinical-grade SARS-CoV-2-specific T-cells from convalescent donors and the ability of these cells to neutralize the virus in vitro, we used blood collected from two COVID-19 convalescent donors (before and after vaccination) that was stimulated with specific SARS-CoV-2 peptides followed by automated T-cell isolation using the CliniMacs Prodigy medical device. To determine cytotoxic activity, HEK 293T cells were transfected to express the SARS-CoV-2 M protein, mimicking SARS-CoV-2 infection. We were able to quickly and efficiently isolate SARS-CoV-2-specific T lymphocytes from both donors before and after they received the Pfizer-BioNTech vaccine. Althoughbefore vaccination, the final product contained up to 7.42% and 30.19% of IFN-γ+ CD3+ T-cells from donor 1 and donor 2, respectively, we observed an enrichment of the IFN-γ+ CD3+ T-cells after vaccination, reaching 70.47% and 42.59%, respectively. At pre-vaccination, the isolated SARS-CoV-2-specific T-cells exhibited cytotoxic activity that was significantly higher than that of unstimulated controls (donor 2: 15.41%, p-value 3.27 × 10-3). The cytotoxic activity of the isolated SARS-CoV-2-specific T-cells also significantly increased after vaccination (donor 1: 32.71%, p-value 1.44 × 10-5; donor 2: 33.38%, p-value 3.13 × 10-6). In conclusion, we demonstrated that SARS-CoV-2-specific T-cells can quickly and efficiently be stimulated from the blood of convalescent donors using SARS-CoV-2-specific peptides followed by automated isolation. Vaccinated convalescent donors have a higher percentage of SARS-CoV-2-specific T-cells and may be more suitable as donors. Although further studies are needed to assess the clinical utility of the functional isolated SARS-CoV-2-specific T-cells in patients, previous studies using the same stimulation and isolation methods applied to other pathologies support this idea.es_ES
dc.description.sponsorshipThis work was supported by MutuaMadrileña Foundation (2020/0056), “Plan Nacional de I+D+I” and Instituto de Salud Carlos III (COVID-19 Research Call COV20/00181), Subdirección General de Redes y Centros de Investigación Cooperativa, Spanish Ministry of Science and Innovation, Spanish Network for Research in Infectious Diseases (REIPI RD16/0016)—co-financed by the European Development Regional Fund (EDRF) and the European Social Fund (ESF) “A way to achieve Europe-The ESF invests in your future “, REACT-EU grant from the Comunidad de Madrid to the ANTICIPA (Complutense University, Madrid). E.G.-R. is supported by the Sara Borrell Program (CD18CIII/00007), Instituto de Salud Carlos III, Ministerio de Ciencia, Innovación y Universidades. F.J.M. is supported by the PFIS Program (F18III/00013), Instituto de Salud Carlos III, Ministerio de Ciencia, Innovación y Universidades.es_ES
dc.language.isoenges_ES
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI) es_ES
dc.type.hasVersionVoRes_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectSARS-CoV-2es_ES
dc.subjectT-lymphocyteses_ES
dc.subjectCytotoxicityes_ES
dc.subjectM proteines_ES
dc.titleIsolation of Functional SARS-CoV-2 Antigen-Specific T-Cells with Specific Viral Cytotoxic Activity for Adoptive Therapy of COVID-19es_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID35327433es_ES
dc.format.volume10es_ES
dc.format.number3es_ES
dc.format.page630es_ES
dc.identifier.doi10.3390/biomedicines10030630es_ES
dc.contributor.funderRed de Investigación Cooperativa en Investigación en Patología Infecciosaes_ES
dc.contributor.funderEuropean Regional Development Fund es_ES
dc.contributor.funderUnión Europea. Fondo Social Europeo (ESF/FSE) es_ES
dc.contributor.funderMinisterio de Ciencia, Innovación y Universidades (España) es_ES
dc.contributor.funderMinisterio de Ciencia e Innovación (España) es_ES
dc.contributor.funderPlan Nacional de I+D+i (España) es_ES
dc.contributor.funderInstituto de Salud Carlos III es_ES
dc.contributor.funderComunidad de Madrid es_ES
dc.contributor.funderFundación Mutua Madrileña es_ES
dc.description.peerreviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.3390/biomedicines10030630es_ES
dc.identifier.journalBiomedicineses_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.rights.accessRightsopen accesses_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/MINECO//RD16%2F0016%2F0004/ES/RED ESPAÑOLA DE INVESTIGACIÓN EN PATOLOGÍAS INFECCIOSAS/ es_ES
dc.relation.projectFISinfo:eu-repo/grantAgreement/ES/COV20/00181es_ES
dc.relation.projectFISinfo:eu-repo/grantAgreement/ES/CD18CIII/00007es_ES
dc.relation.projectFISinfo:eu-repo/grantAgreement/ES/F18III/00013es_ES


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