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dc.contributor.author | Bleriot, Ines | |
dc.contributor.author | Blasco, Lucia | |
dc.contributor.author | Pacios, Olga | |
dc.contributor.author | Fernández-García, Laura | |
dc.contributor.author | Ambroa, Antón | |
dc.contributor.author | López, María | |
dc.contributor.author | Ortiz-Cartagena, Concha | |
dc.contributor.author | Cuenca, Felipe Fernández | |
dc.contributor.author | Oteo-Iglesias, Jesus | |
dc.contributor.author | Pascual, Álvaro | |
dc.contributor.author | Martínez-Martínez, Luis | |
dc.contributor.author | Domingo-Calap, Pilar | |
dc.contributor.author | Wood, Thomas K | |
dc.contributor.author | Tomás, María | |
dc.contributor.author | Wood, Thomas K. | |
dc.date.accessioned | 2022-05-24T07:09:05Z | |
dc.date.available | 2022-05-24T07:09:05Z | |
dc.date.issued | 2022-03-16 | |
dc.identifier.citation | Sci Rep. 2022 Mar 16;12(1):4488. | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/14469 | |
dc.description.abstract | Since their discovery, toxin-antitoxin (TA) systems have captivated the attention of many scientists. Recent studies have demonstrated that TA systems play a key role in phage inhibition. The aim of the present study was to investigate the role of the PemIK (PemK/PemI) type II TA system in phage inhibition by its intrinsic expression in clinical strains of Klebsiella pneumoniae carrying the lncL plasmid, which harbours the carbapenemase OXA-48 and the PemK/PemI TA system. Furthermore, induced expression of the system in an IPTG-inducible plasmid in a reference strain of K. pneumoniae ATCC10031 was also studied. The results showed that induced expression of the whole TA system did not inhibit phage infection, whereas overexpression of the pemK toxin prevented early infection. To investigate the molecular mechanism involved in the PemK toxin-mediated inhibition of phage infection, assays measuring metabolic activity and viability were performed, revealing that overexpression of the PemK toxin led to dormancy of the bacteria. Thus, we demonstrate that the PemK/PemI TA system plays a role in phage infection and that the action of the free toxin induces a dormant state in the cells, resulting in inhibition of phage infections. | es_ES |
dc.description.sponsorship | This study was funded by grant PI19/00878 awarded to M. Tomás within the State Plan for R+D+I 2013-2016 (National Plan for Scientific Research, Technological Development and Innovation 2008-2011) and co-financed by the ISCIII-Deputy General Directorate for Evaluation and Promotion of Research—European Regional Development Fund "A way of Making Europe" and Instituto de Salud Carlos III FEDER, Spanish Network for the Research in Infectious Diseases (REIPI, RD16/0016/0001, RD16/0016/0006 and RD16/CIII/0004/0002) and by the Study Group on Mechanisms of Action and Resistance to Antimicrobials, GEMARA (SEIMC, http://www.seimc.org/). M. Tomás was financially supported by the Miguel Servet Research Programme (SERGAS and ISCIII). I. Bleriot was financially supported by pFIS program (ISCIII, FI20/00302). O. Pacios and M. López was financially supported by a grant IN606A-2020/035 and IN606B-2018/008, respectively (GAIN, Xunta de Galicia) and M. Gonzalez-Bardanca was financially supported by the Rio Hortega program (ISCIII, CM20/00198). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Nature Publishing Group | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Developmental biology | es_ES |
dc.subject | Microbiology | es_ES |
dc.subject | Molecular biology | es_ES |
dc.subject | Molecular medicine | es_ES |
dc.title | The role of PemIK (PemK/PemI) type II TA system from Klebsiella pneumoniae clinical strains in lytic phage infection | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Atribución 4.0 Internacional | * |
dc.identifier.pubmedID | 35296704 | es_ES |
dc.format.volume | 12 | es_ES |
dc.format.number | 1 | es_ES |
dc.format.page | 4488 | es_ES |
dc.identifier.doi | 10.1038/s41598-022-08111-5 | es_ES |
dc.contributor.funder | Instituto de Salud Carlos III | es_ES |
dc.contributor.funder | Plan Nacional de I+D+i (España) | es_ES |
dc.contributor.funder | Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) | es_ES |
dc.contributor.funder | RETICS-Investigación en Patología Infecciosa (REIPI-ISCIII) (España) | es_ES |
dc.contributor.funder | Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica | es_ES |
dc.contributor.funder | Xunta de Galicia (España) | es_ES |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 2045-2322 | es_ES |
dc.relation.publisherversion | https://doi.org/10.1038/s41598-022-08111-5 | es_ES |
dc.identifier.journal | Scientific Reports | es_ES |
dc.repisalud.centro | ISCIII::Centro Nacional de Microbiología | es_ES |
dc.repisalud.institucion | ISCIII | es_ES |
dc.rights.accessRights | open access | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/MINECO//RD16%2F0016%2F0001/ES/RED ESPAÑOLA DE INVESTIGACIÓN EN PATOLOGÍAS INFECCIOSAS/ | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/MINECO//RD16%2F0016%2F0006/ES/RED ESPAÑOLA DE INVESTIGACIÓN EN PATOLOGÍAS INFECCIOSAS/ | es_ES |
dc.relation.projectFIS | info:eu-repo/grantAgreement/ES/RD16/CIII/0004/0002 | es_ES |
dc.relation.projectFIS | info:eu-repo/grantAgreement/ES/FI20/00302 | es_ES |
dc.relation.projectFIS | info:eu-repo/grantAgreement/ES/CM20/00198 | es_ES |
dc.relation.projectFIS | info:eu-repo/grantAgreement/ES/PI19/00878 | es_ES |