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dc.contributor.authorTa Tang, Thuy-Huong 
dc.contributor.authorBerzosa, Pedro 
dc.contributor.authorRubio Gonzalez, Jose Miguel 
dc.contributor.authorRomay-Barja, Maria 
dc.contributor.authorNcogo, Policarpo
dc.contributor.authorAgudo, Diego
dc.contributor.authorHerrador, Zaida 
dc.contributor.authorCerrada-Gálvez, Laura
dc.contributor.authorBenito, Agustin 
dc.date.accessioned2022-05-23T09:42:21Z
dc.date.available2022-05-23T09:42:21Z
dc.date.issued2022-02-11
dc.identifier.citationMem Inst Oswaldo Cruz. 2022 Feb 11;116:e210210.es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/14449
dc.description.abstractBackground: Loa loa is a filarial species found exclusively in West and Central Africa. Microscopy is the traditional diagnosis method for human loiasis. Several molecular methods have developed as an alternative approach for identification of L. loa filarial parasites. Objectives: The aim of this study was to evaluate a Loa-Loop-mediated isothermal amplification (LAMP) assay to diagnose loiasis disease on dried blood spots (DBS) samples, compared to microscopy, filaria-real time-polymerase chain reaction (PCR) and nested-Loa PCR. Methods: A total of 100 DBS samples and 100 blood smears were used for this study. DNA was extracted using saponin/Chelex method. DNA isolated was assayed by a Loa-LAMP assay in parallel to microscopy, filaria-real time PCR and nested-Loa PCR. The sensitivities and specificities of Loa-LAMP assay was computed comparing to each one of the reference methods. Findings: Loa-LAMP's sensitivity was more than 90% and specificity was nearly 100% when compared to molecular methods. On the other hand, sensitivity was decreased a bit when Loa-LAMP faced microscopy, but keeping the other statistical values high. Main conclusions: Loa-LAMP is an appropriate method for loiasis diagnosis in endemic areas. Though, it has disadvantages like the reagents' high price at the moment and not to be able to detect more filarial species at once.es_ES
dc.description.sponsorshipFinancial support: Fundación Estatal, Salud, Infancia y Bienestar Social (Institute of Health Carlos III) and Cooperative Research Network on Tropical Diseases. T-HT-T is hired at the Malaria and Neglected Tropical Diseases Laboratory of National Centre of Tropical Medicine with a contract named ISCIII-SARA BORRELL CD17CIII/00018, financed by the Institute of Health Carlos III.es_ES
dc.language.isoenges_ES
dc.publisherFundação Oswaldo Cruz (Fiocruz) es_ES
dc.type.hasVersionVoRes_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectFilariaees_ES
dc.subjectLoa loaes_ES
dc.subjectDBSes_ES
dc.subjectMolecular diagnosises_ES
dc.subjectLAMPes_ES
dc.subjectreal-time PCRes_ES
dc.subject.meshLoiasis es_ES
dc.subject.meshHumans es_ES
dc.subject.meshMicroscopy es_ES
dc.subject.meshMolecular Diagnostic Techniques es_ES
dc.subject.meshNucleic Acid Amplification Techniques es_ES
dc.subject.meshReal-Time Polymerase Chain Reaction es_ES
dc.subject.meshSensitivity and Specificity es_ES
dc.titleEvaluation of LAMP for the diagnosis of Loa loa infection in dried blood spots compared to PCR-based assays and microscopyes_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID35170676es_ES
dc.format.volume116es_ES
dc.format.pagee210210es_ES
dc.identifier.doi10.1590/0074-02760210210es_ES
dc.contributor.funderInstituto de Salud Carlos III es_ES
dc.contributor.funderRETICS-Investigación colaborativa en Enfermedades Tropicales (RICET-ISCIII) (España) es_ES
dc.description.peerreviewedes_ES
dc.identifier.e-issn1678-8060es_ES
dc.relation.publisherversionhttps://doi.org/10.1590/0074-02760210210es_ES
dc.identifier.journalMemorias do Instituto Oswaldo Cruzes_ES
dc.repisalud.centroISCIII::Centro Nacional de Medicina Tropicales_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiología
dc.repisalud.institucionISCIIIes_ES
dc.rights.accessRightsopen accesses_ES
dc.relation.projectFISinfo:eu-repo/grantAgreement/ES/CD17CIII/00018es_ES


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Atribución 4.0 Internacional
Este Item está sujeto a una licencia Creative Commons: Atribución 4.0 Internacional