Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/14440
Título
Plasma miRNA profile at COVID-19 onset predicts severity status and mortality
Autor(es)
Fernández-Pato, Asier | Virseda-Berdices, Ana ISCIII | Resino, Salvador ISCIII | Ryan, Pablo | Martínez-González, Oscar | Peréz-García, Felipe | Martin-Vicente, Maria ISCIII | Valle-Millares, Daniel ISCIII | Brochado-Kith, Oscar ISCIII | Blancas, Rafael | Martínez, Amalia | Ceballos, Francisco C ISCIII | Bartolomé-Sánchez, Sofía ISCIII | Vidal-Alcántara, Erick Joan ISCIII | Alonso, David | Blanca-López, Natalia | Martinez-Acitores, Ignacio Ramirez | Martin-Pedraza, Laura | Jimenez-Sousa, Maria Angeles ISCIII | Fernandez-Rodriguez, Amanda ISCIII
Fecha de publicación
2022-02-27
Cita
Emerg Microbes Infect. 2022;11(1):676-688.
Idioma
Inglés
Tipo de documento
journal article
Resumen
Background: MicroRNAs (miRNAs) have a crucial role in regulating immune response against infectious diseases, showing changes early in disease onset and before the detection of the pathogen. Thus, we aimed to analyze the plasma miRNA profile at COVID-19 onset to identify miRNAs as early prognostic biomarkers of severity and survival. Methods and results: Plasma miRNome of 96 COVID-19 patients that developed asymptomatic/mild, moderate and severe disease was sequenced together with a group of healthy controls. Plasma immune-related biomarkers were also assessed. COVID-19 patients showed 200 significant differentially expressed (SDE) miRNAs concerning healthy controls, with upregulated putative targets of SARS-CoV-2, and inflammatory miRNAs. Among COVID-19 patients, 75 SDE miRNAs were observed in asymptomatic/mild compared to symptomatic patients, which were involved in platelet aggregation and cytokine pathways, among others. Moreover, 137 SDE miRNAs were identified between severe and moderate patients, where miRNAs targeting the SARS CoV-2 genome were the most strongly disrupted. Finally, we constructed a mortality predictive risk score (miRNA-MRS) with ten miRNAs. Patients with higher values had a higher risk of 90-days mortality (hazard ratio = 4.60; p-value < 0.001). Besides, the discriminant power of miRNA-MRS was significantly higher than the observed for age and gender (AUROC = 0.970 vs. 0.881; p = 0.042). Conclusions: SARS-CoV-2 infection deeply disturbs the plasma miRNome from an early stage of COVID-19, making miRNAs highly valuable as early predictors of severity and mortality.
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