dc.contributor.author | Bacqué, Joan | |
dc.contributor.author | Delgado, Elena | |
dc.contributor.author | Benito, Sonia | |
dc.contributor.author | Moreno-Lorenzo, María | |
dc.contributor.author | Montero, Vanessa | |
dc.contributor.author | Gil, Horacio | |
dc.contributor.author | Sanchez-Martinez, Monica | |
dc.contributor.author | Nieto-Toboso, María Carmen | |
dc.contributor.author | Muñoz, Josefa | |
dc.contributor.author | Zubero-Sulibarria, Miren Z | |
dc.contributor.author | Ugalde, Estíbaliz | |
dc.contributor.author | Garcia-Bodas, Elena | |
dc.contributor.author | Cañada-Garcia, Javier Enrique | |
dc.contributor.author | Del Romero, Jorge | |
dc.contributor.author | Rodríguez, Carmen | |
dc.contributor.author | Rodríguez-Avial, Iciar | |
dc.contributor.author | Elorduy-Otazua, Luis | |
dc.contributor.author | Portu, José J | |
dc.contributor.author | García-Costa, Juan | |
dc.contributor.author | Ocampo, Antonio | |
dc.contributor.author | Cabrera, Jorge Julio | |
dc.contributor.author | Thomson, Michael M | |
dc.date.accessioned | 2022-05-09T10:04:55Z | |
dc.date.available | 2022-05-09T10:04:55Z | |
dc.date.issued | 2021-11 | |
dc.identifier.citation | Front Microbiol. 2021 Nov 15;12:774386. | es_ES |
dc.identifier.issn | 1664-302X | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/14326 | |
dc.description.abstract | Circulating recombinant forms (CRFs) are important components of the HIV-1 pandemic. Among 110 reported in the literature, 17 are BF1 intersubtype recombinant, most of which are of South American origin. Among these, all 5 identified in the Southern Cone and neighboring countries, except Brazil, derive from a common recombinant ancestor related to CRF12_BF, which circulates widely in Argentina, as deduced from coincident breakpoints and clustering in phylogenetic trees. In a HIV-1 molecular epidemiological study in Spain, we identified a phylogenetic cluster of 20 samples from 3 separate regions which were of F1 subsubtype, related to the Brazilian strain, in protease-reverse transcriptase (Pr-RT) and of subtype B in integrase. Remarkably, 14 individuals from this cluster (designated BF9) were Paraguayans and only 4 were native Spaniards. HIV-1 transmission was predominantly heterosexual, except for a subcluster of 6 individuals, 5 of which were men who have sex with men. Ten additional database sequences, from Argentina (n = 4), Spain (n = 3), Paraguay (n = 1), Brazil (n = 1), and Italy (n = 1), branched within the BF9 cluster. To determine whether it represents a new CRF, near full-length genome (NFLG) sequences were obtained for 6 viruses from 3 Spanish regions. Bootscan analyses showed a coincident BF1 recombinant structure, with 5 breakpoints, located in p17 gag , integrase, gp120, gp41-rev overlap, and nef, which was identical to that of two BF1 recombinant viruses from Paraguay previously sequenced in NFLGs. Interestingly, none of the breakpoints coincided with those of CRF12_BF. In a maximum likelihood phylogenetic tree, all 8 NFLG sequences grouped in a strongly supported clade segregating from previously identified CRFs and from the CRF12_BF "family" clade. These results allow us to identify a new HIV-1 CRF, designated CRF66_BF. Through a Bayesian coalescent analysis, the most recent common ancestor of CRF66_BF was estimated around 1984 in South America, either in Paraguay or Argentina. Among Pr-RT sequences obtained by us from HIV-1-infected Paraguayans living in Spain, 14 (20.9%) of 67 were of CRF66_BF, suggesting that CRF66_BF may be one of the major HIV-1 genetic forms circulating in Paraguay. CRF66_BF is the first reported non-Brazilian South American HIV-1 CRF_BF unrelated to CRF12_BF. | es_ES |
dc.description.sponsorship | This work was funded through Acción Estratégica en Salud Intramural (AESI), Instituto de Salud Carlos III, projects PI16CIII/00033 and PI19CIII/00042; Red de Investigación en SIDA (RIS), Instituto de Salud Carlos III, Subdirección General de Evaluación y Fondo Europeo de Desarrollo Regional (FEDER), Plan Nacional I+D+I, project RD16ISCIII/0002/0004; and scientific agreements with Consellería de Sanidade, Government of Galicia (MVI 1004/16) and Osakidetza-Servicio Vasco de Salud, Government of Basque Country (MVI 1001/16). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Frontiers Media | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | HIV-1 | es_ES |
dc.subject | Circulating recombinant form | es_ES |
dc.subject | Molecular epidemiology | es_ES |
dc.subject | Phylodynamics | es_ES |
dc.subject | Phylogeny | es_ES |
dc.title | Identification of CRF66_BF, a New HIV-1 Circulating Recombinant Form of South American Origin | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Atribución 4.0 Internacional | * |
dc.identifier.pubmedID | 34867914 | es_ES |
dc.format.volume | 12 | es_ES |
dc.format.page | 774386 | es_ES |
dc.identifier.doi | 10.3389/fmicb.2021.774386 | es_ES |
dc.contributor.funder | Instituto de Salud Carlos III | es_ES |
dc.contributor.funder | Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) | es_ES |
dc.contributor.funder | Plan Nacional de I+D+i (España) | es_ES |
dc.contributor.funder | Red de Investigación Cooperativa en Investigación en Sida (España) | es_ES |
dc.contributor.funder | Xunta de Galicia (España) | es_ES |
dc.contributor.funder | Basque Government (España) | es_ES |
dc.description.peerreviewed | Sí | es_ES |
dc.relation.publisherversion | https://doi.org/10.3389/fmicb.2021.774386 | es_ES |
dc.identifier.journal | Frontiers in Microbiology | es_ES |
dc.repisalud.centro | ISCIII::Centro Nacional de Microbiología | es_ES |
dc.repisalud.institucion | ISCIII | es_ES |
dc.rights.accessRights | open access | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/RD16ISCIII/0002/0004 | es_ES |
dc.relation.projectFIS | info:fis/Instituto de Salud Carlos III/Programa Estatal de Fomento de la Investigación Científica y Técnica de Excelencia/Subprograma Estatal de Generación de Conocimiento/ISCIII 2016 Modalidad Proyectos de Investigacion en Salud Intramurales. (2016)/PI16CIII/00033 | es_ES |
dc.relation.projectFIS | info:fis/Instituto de Salud Carlos III/Programa Estatal de Generación de Conocimiento y Fortalecimiento del Sistema Español de I+D+I/Subprograma Estatal de Generación de Conocimiento/PI19-ISCIII Modalidad Proyectos de Investigacion en Salud Intramurales. (2019)/PI19CIII/00042 | es_ES |