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dc.contributor.author | Ortega-García, María Victoria | |
dc.contributor.author | Salguero, Francisco Javier | |
dc.contributor.author | García, Nerea | |
dc.contributor.author | Dominguez-Rodriguez, Mercedes | |
dc.contributor.author | Moreno-Iruela, Inmaculada | |
dc.contributor.author | Berrocal, Alexis | |
dc.date.accessioned | 2022-04-22T15:31:45Z | |
dc.date.available | 2022-04-22T15:31:45Z | |
dc.date.issued | 2021-08-02 | |
dc.identifier.citation | Vet Med Sci. 2021; Aug. 1-6 | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/14159 | |
dc.description.abstract | Background: Cutaneous forms of leishmaniosis due to Leishmania braziliensis have been reported in horses in the New World. Domestic animals play a role in the transmission of the disease. In Costa Rica, human cases of L. braziliensis, L. panamensis and L. infantum have been reported. Objectives: The present report describes five cases of equine cutaneous leishmaniosis in Costa Rica. The aetiological diagnosis was based on the presence of the parasite within the lesions. Methods: Skin biopsies were used to perform histopathological analyses of the lesions. Immunohistochemistry was used to detect the presence of the Leishmania spp. antigens in tissue sections. Laser-capture micro-dissection and quantitative real-time PCR techniques were carried out to detect the pathogen nucleic acid within the microscopic lesions. Results: Histopathological analyses showed a granulomatous inflammation within the dermis, with multi-nucleated giant cells, macrophages, lymphocytes and few neutrophils and eosinophils. We detected the parasite by immunohistochemistry, using a rabbit polyclonal antibody raised against Leishmania spp. However, we could not identify Leishmania spp. by quantitative real-time PCR in formalin-fixed paraffin-embedded tissues, using specific primers for the conserved region in the minicircle of the Leishmania DNA kinetoplast. Conclusions: Our results emphasise the importance of Leishmania spp. not only as a causative agent of equine cutaneous disease in the New World, but also as a possible emerging pathogen. Leishmaniosis is one of the most prevalent parasitic public health problems worldwide, and equines may have a role in the epidemiology of the disease. | es_ES |
dc.description.sponsorship | This study was supported by the Ayuntamiento de Madrid of the Comunidad de Madrid S2013/ABI-2747 (TAVS-CM) and by the Structural Funds of the European Union. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Wiley | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Leishmania spp | es_ES |
dc.subject | Cutaneous leishmaniosis | es_ES |
dc.subject | Histopathology | es_ES |
dc.subject | Horse | es_ES |
dc.subject | Immunohistochemistry | es_ES |
dc.subject | Leishmaniosis | es_ES |
dc.title | Equine infection with Leishmania spp. in Costa Rica: Study of five cases | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.identifier.pubmedID | 34342158 | es_ES |
dc.format.volume | 7 | es_ES |
dc.format.number | 6 | es_ES |
dc.format.page | 2234-2239 | es_ES |
dc.identifier.doi | 10.1002/vms3.587 | es_ES |
dc.contributor.funder | Ayuntamiento de Madrid (España) | es_ES |
dc.contributor.funder | Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) | es_ES |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 2053-1095 | es_ES |
dc.relation.publisherversion | https://doi.org/10.1002/vms3.587 | es_ES |
dc.identifier.journal | Veterinary Medicine and Science | es_ES |
dc.repisalud.centro | ISCIII::Centro Nacional de Microbiología | es_ES |
dc.repisalud.institucion | ISCIII | es_ES |
dc.rights.accessRights | open access | es_ES |