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dc.contributor.authorOrtega-García, María Victoria
dc.contributor.authorSalguero, Francisco Javier
dc.contributor.authorGarcía, Nerea
dc.contributor.authorDominguez-Rodriguez, Mercedes 
dc.contributor.authorMoreno-Iruela, Inmaculada 
dc.contributor.authorBerrocal, Alexis
dc.date.accessioned2022-04-22T15:31:45Z
dc.date.available2022-04-22T15:31:45Z
dc.date.issued2021-08-02
dc.identifier.citationVet Med Sci. 2021; Aug. 1-6es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/14159
dc.description.abstractBackground: Cutaneous forms of leishmaniosis due to Leishmania braziliensis have been reported in horses in the New World. Domestic animals play a role in the transmission of the disease. In Costa Rica, human cases of L. braziliensis, L. panamensis and L. infantum have been reported. Objectives: The present report describes five cases of equine cutaneous leishmaniosis in Costa Rica. The aetiological diagnosis was based on the presence of the parasite within the lesions. Methods: Skin biopsies were used to perform histopathological analyses of the lesions. Immunohistochemistry was used to detect the presence of the Leishmania spp. antigens in tissue sections. Laser-capture micro-dissection and quantitative real-time PCR techniques were carried out to detect the pathogen nucleic acid within the microscopic lesions. Results: Histopathological analyses showed a granulomatous inflammation within the dermis, with multi-nucleated giant cells, macrophages, lymphocytes and few neutrophils and eosinophils. We detected the parasite by immunohistochemistry, using a rabbit polyclonal antibody raised against Leishmania spp. However, we could not identify Leishmania spp. by quantitative real-time PCR in formalin-fixed paraffin-embedded tissues, using specific primers for the conserved region in the minicircle of the Leishmania DNA kinetoplast. Conclusions: Our results emphasise the importance of Leishmania spp. not only as a causative agent of equine cutaneous disease in the New World, but also as a possible emerging pathogen. Leishmaniosis is one of the most prevalent parasitic public health problems worldwide, and equines may have a role in the epidemiology of the disease.es_ES
dc.description.sponsorshipThis study was supported by the Ayuntamiento de Madrid of the Comunidad de Madrid S2013/ABI-2747 (TAVS-CM) and by the Structural Funds of the European Union.es_ES
dc.language.isoenges_ES
dc.publisherWiley es_ES
dc.type.hasVersionVoRes_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectLeishmania sppes_ES
dc.subjectCutaneous leishmaniosises_ES
dc.subjectHistopathologyes_ES
dc.subjectHorsees_ES
dc.subjectImmunohistochemistryes_ES
dc.subjectLeishmaniosises_ES
dc.titleEquine infection with Leishmania spp. in Costa Rica: Study of five caseses_ES
dc.typejournal articlees_ES
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.identifier.pubmedID34342158es_ES
dc.format.volume7es_ES
dc.format.number6es_ES
dc.format.page2234-2239es_ES
dc.identifier.doi10.1002/vms3.587es_ES
dc.contributor.funderAyuntamiento de Madrid (España) es_ES
dc.contributor.funderUnión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) es_ES
dc.description.peerreviewedes_ES
dc.identifier.e-issn2053-1095es_ES
dc.relation.publisherversionhttps://doi.org/10.1002/vms3.587es_ES
dc.identifier.journalVeterinary Medicine and Sciencees_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.rights.accessRightsopen accesses_ES


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
This item is licensed under a: Attribution-NonCommercial-NoDerivatives 4.0 Internacional