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dc.contributor.authorLozano-Prieto, Marta
dc.contributor.authorAdlam, David
dc.contributor.authorGarcia-Guimaraes, Marcos
dc.contributor.authorSanz-Garcia, Ancor
dc.contributor.authorVera-Tome, Paula
dc.contributor.authorRivero, Fernando
dc.contributor.authorCuesta, Javier
dc.contributor.authorBastante, Teresa
dc.contributor.authorBaranowska-Clarke, Anna A
dc.contributor.authorVara, Alicia
dc.contributor.authorMartin-Gayo, Enrique
dc.contributor.authorVicente-Manzanares, Miguel
dc.contributor.authorMartin, Pilar 
dc.contributor.authorSamani, Nilesh J
dc.contributor.authorSanchez-Madrid, Francisco 
dc.contributor.authorAlfonso, Fernando
dc.contributor.authorde la Fuente, Hortensia 
dc.date.accessioned2021-10-04T08:34:55Z
dc.date.available2021-10-04T08:34:55Z
dc.date.issued2021-04
dc.identifier.citationEBioMedicine. 2021; 66:103338es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/13422
dc.description.abstractSpontaneous Coronary Artery Dissection (SCAD) is an important cause of acute coronary syndromes, particularly in young to middle-aged women. Differentiating acute SCAD from coronary atherothrombosis remains a major clinical challenge. A case-control study was used to explore the usefulness of circulating miRNAs to discriminate both clinical entities. The profile of miRNAs was evaluated using an unbiased human RT-PCR platform and confirmed using individual primers. miRNAs were evaluated in plasma samples from acute SCAD and atherothrombotic acute myocardial infarction (AT-AMI) from two independent cohorts; discovery cohort (SCAD n = 15, AT-AMI n = 15), and validation cohort (SCAD n = 11, AT-AMI n = 41) with 9 healthy control subjects. Plasma levels of IL-8, TGFB1, TGBR1, Endothelin-1 and MMP2 were analysed by ELISA assays. From 15 differentially expressed miRNAs detected in cohort 1, we confirmed in cohort 2 the differential expression of 4 miRNAs: miR-let-7f-5p, miR-146a-5p, miR-151a-3p and miR-223-5p, whose expression was higher in SCAD compared to AT-AMI. The combined expression of these 4 miRNAs showed the best predictive value to distinguish between both entities (AUC: 0.879, 95% CI 0.72-1.0) compared to individual miRNAs. Functional profiling of target genes identified an association with blood vessel biology, TGF-beta pathway and cytoskeletal traction force. ELISA assays showed high plasma levels of IL-8, TGFB1, TGFBR1, Endothelin-1 and MMP2 in SCAD patients compared to AT-AMI. We present a novel signature of plasma miRNAs in patients with SCAD. miR-let-7f-5p, miR-146a-5p, miR-151a-3p and miR-223-5p discriminate SCAD from AT-AMI patients and also shed light on the pathological mechanisms underlying this condition. Spanish Ministry of Economy and Competitiveness (MINECO): Plan Nacional de Salud SAF2017-82886-R, Centro de Investigación Biomédica en Red de Enfermedades Cardiovasculares (CIBERCV). Fundación BBVA a equipos de Investigación Científica 2018 and from Caixa Banking Foundation under the project code HR17-00016 to F.S.M. Instituto de Salud Carlos III (AES 2019): PI19/00565 to F.R, PI19/00545 to P.M. CAM (S2017/BMD-3671-INFLAMUNE-CM) from Comunidad de Madrid to FSM and PM. The UK SCAD study was supported by BeatSCAD, the British Heart Foundation (BHF) PG/13/96/30608 the NIHR rare disease translational collaboration and the Leicester NIHR Biomedical Research Centre.es_ES
dc.description.sponsorshipSpanish Ministry of Economy and Competitiveness (MINECO): Plan Nacional de Salud SAF2017-82886-R, Centro de Investigación Biomédica en Red de Enfermedades Cardiovasculares (CIBERCV). Fundación BBVA a equipos de Investigación Científica 2018 and from Caixa Banking Foundation under the project code HR17-00016 to F.S.M. Instituto de Salud Carlos III (AES 2019): PI19/00565 to F.R, PI19/00545 to P.M. CAM (S2017/BMD-3671-INFLAMUNE-CM) from Comunidad de Madrid to FSM and PM. The UK SCAD study was supported by BeatSCAD, the British Heart Foundation (BHF) PG/13/96/30608 the NIHR rare disease translational collaboration and the Leicester NIHR Biomedical Research Centre.es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleDifferential miRNAs in acute spontaneous coronary artery dissection: Pathophysiological insights from a potential biomarker.es_ES
dc.typeArtículoes_ES
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.identifier.pubmedID33866193es_ES
dc.format.volume66es_ES
dc.format.page103338es_ES
dc.identifier.doi10.1016/j.ebiom.2021.103338es_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)
dc.contributor.funderCentro de Investigación Biomedica en Red - CIBER
dc.contributor.funderFundación BBVA
dc.contributor.funderInstituto de Salud Carlos III - ISCIII
dc.contributor.funderComunidad de Madrid
dc.contributor.funderBritish Heart Foundation
dc.contributor.funderLeicester NIHR Biomedical Research Centre
dc.description.peerreviewedes_ES
dc.identifier.e-issn2352-3964es_ES
dc.relation.publisherversionhttps://doi.org/10.1016/j.ebiom.2021.103338es_ES
dc.identifier.journalEBioMedicinees_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Comunicación Intercelular en la Respuesta Inflamatoriaes_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Moléculas Reguladoras de los Procesos Inflamatorioses_ES
dc.repisalud.institucionCNICes_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/SAF2017-82886-Res_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/HR17-00016es_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/PI19/00565es_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/PI19/00545es_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/S2017/BMD-3671-INFLAMUNE-CMes_ES


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
This item is licensed under a: Attribution-NonCommercial-NoDerivatives 4.0 Internacional