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dc.contributor.author | Lozano-Prieto, Marta | |
dc.contributor.author | Adlam, David | |
dc.contributor.author | Garcia-Guimaraes, Marcos | |
dc.contributor.author | Sanz-Garcia, Ancor | |
dc.contributor.author | Vera-Tome, Paula | |
dc.contributor.author | Rivero, Fernando | |
dc.contributor.author | Cuesta, Javier | |
dc.contributor.author | Bastante, Teresa | |
dc.contributor.author | Baranowska-Clarke, Anna A | |
dc.contributor.author | Vara, Alicia | |
dc.contributor.author | Martin-Gayo, Enrique | |
dc.contributor.author | Vicente-Manzanares, Miguel | |
dc.contributor.author | Martin, Pilar | |
dc.contributor.author | Samani, Nilesh J | |
dc.contributor.author | Sanchez-Madrid, Francisco | |
dc.contributor.author | Alfonso, Fernando | |
dc.contributor.author | de la Fuente, Hortensia | |
dc.date.accessioned | 2021-10-04T08:34:55Z | |
dc.date.available | 2021-10-04T08:34:55Z | |
dc.date.issued | 2021-04 | |
dc.identifier.citation | EBioMedicine. 2021; 66:103338 | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/13422 | |
dc.description.abstract | Spontaneous Coronary Artery Dissection (SCAD) is an important cause of acute coronary syndromes, particularly in young to middle-aged women. Differentiating acute SCAD from coronary atherothrombosis remains a major clinical challenge. A case-control study was used to explore the usefulness of circulating miRNAs to discriminate both clinical entities. The profile of miRNAs was evaluated using an unbiased human RT-PCR platform and confirmed using individual primers. miRNAs were evaluated in plasma samples from acute SCAD and atherothrombotic acute myocardial infarction (AT-AMI) from two independent cohorts; discovery cohort (SCAD n = 15, AT-AMI n = 15), and validation cohort (SCAD n = 11, AT-AMI n = 41) with 9 healthy control subjects. Plasma levels of IL-8, TGFB1, TGBR1, Endothelin-1 and MMP2 were analysed by ELISA assays. From 15 differentially expressed miRNAs detected in cohort 1, we confirmed in cohort 2 the differential expression of 4 miRNAs: miR-let-7f-5p, miR-146a-5p, miR-151a-3p and miR-223-5p, whose expression was higher in SCAD compared to AT-AMI. The combined expression of these 4 miRNAs showed the best predictive value to distinguish between both entities (AUC: 0.879, 95% CI 0.72-1.0) compared to individual miRNAs. Functional profiling of target genes identified an association with blood vessel biology, TGF-beta pathway and cytoskeletal traction force. ELISA assays showed high plasma levels of IL-8, TGFB1, TGFBR1, Endothelin-1 and MMP2 in SCAD patients compared to AT-AMI. We present a novel signature of plasma miRNAs in patients with SCAD. miR-let-7f-5p, miR-146a-5p, miR-151a-3p and miR-223-5p discriminate SCAD from AT-AMI patients and also shed light on the pathological mechanisms underlying this condition. Spanish Ministry of Economy and Competitiveness (MINECO): Plan Nacional de Salud SAF2017-82886-R, Centro de Investigación Biomédica en Red de Enfermedades Cardiovasculares (CIBERCV). Fundación BBVA a equipos de Investigación Científica 2018 and from Caixa Banking Foundation under the project code HR17-00016 to F.S.M. Instituto de Salud Carlos III (AES 2019): PI19/00565 to F.R, PI19/00545 to P.M. CAM (S2017/BMD-3671-INFLAMUNE-CM) from Comunidad de Madrid to FSM and PM. The UK SCAD study was supported by BeatSCAD, the British Heart Foundation (BHF) PG/13/96/30608 the NIHR rare disease translational collaboration and the Leicester NIHR Biomedical Research Centre. | es_ES |
dc.description.sponsorship | Spanish Ministry of Economy and Competitiveness (MINECO): Plan Nacional de Salud SAF2017-82886-R, Centro de Investigación Biomédica en Red de Enfermedades Cardiovasculares (CIBERCV). Fundación BBVA a equipos de Investigación Científica 2018 and from Caixa Banking Foundation under the project code HR17-00016 to F.S.M. Instituto de Salud Carlos III (AES 2019): PI19/00565 to F.R, PI19/00545 to P.M. CAM (S2017/BMD-3671-INFLAMUNE-CM) from Comunidad de Madrid to FSM and PM. The UK SCAD study was supported by BeatSCAD, the British Heart Foundation (BHF) PG/13/96/30608 the NIHR rare disease translational collaboration and the Leicester NIHR Biomedical Research Centre. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Differential miRNAs in acute spontaneous coronary artery dissection: Pathophysiological insights from a potential biomarker. | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.identifier.pubmedID | 33866193 | es_ES |
dc.format.volume | 66 | es_ES |
dc.format.page | 103338 | es_ES |
dc.identifier.doi | 10.1016/j.ebiom.2021.103338 | es_ES |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | |
dc.contributor.funder | Centro de Investigación Biomedica en Red - CIBER | |
dc.contributor.funder | Fundación BBVA | |
dc.contributor.funder | Instituto de Salud Carlos III | |
dc.contributor.funder | Comunidad de Madrid (España) | |
dc.contributor.funder | British Heart Foundation | |
dc.contributor.funder | NIHR - Leicester Biomedical Research Centre (Reino Unido) | |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 2352-3964 | es_ES |
dc.relation.publisherversion | https://doi.org/10.1016/j.ebiom.2021.103338 | es_ES |
dc.identifier.journal | EBioMedicine | es_ES |
dc.repisalud.orgCNIC | CNIC::Grupos de investigación::Comunicación Intercelular en la Respuesta Inflamatoria | es_ES |
dc.repisalud.orgCNIC | CNIC::Grupos de investigación::Moléculas Reguladoras de los Procesos Inflamatorios | es_ES |
dc.repisalud.institucion | CNIC | es_ES |
dc.rights.accessRights | open access | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/SAF2017-82886-R | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/HR17-00016 | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/PI19/00565 | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/PI19/00545 | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/S2017/BMD-3671-INFLAMUNE-CM | es_ES |