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dc.contributor.author | Loras, Cristina | |
dc.contributor.author | González-Prieto, Andrea | |
dc.contributor.author | Perez-Vazquez, Maria | |
dc.contributor.author | Bautista, Veronica | |
dc.contributor.author | Ávila, Alicia | |
dc.contributor.author | Sola-Campoy, Pedro Juan | |
dc.contributor.author | Oteo-Iglesias, Jesus | |
dc.contributor.author | Alós, Juan-Ignacio | |
dc.date.accessioned | 2021-05-17T09:19:06Z | |
dc.date.available | 2021-05-17T09:19:06Z | |
dc.date.issued | 2021-03-21 | |
dc.identifier.citation | J Glob Antimicrob Resist . 2021 Mar 21;25:137-141. | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/12965 | |
dc.description.abstract | The aim of this this study was to describe the presence of different variants of the fosA gene in fosfomycin-resistant Escherichia coli strains in Madrid, Spain. fos genes were searched for in 55 E. coli strains collected from seven representative hospitals located in Madrid. A phenotypic screening test was performed following the disk diffusion method with sodium phosphonoformate added as described by Nakamura et al. Additionally, a molecular study based on PCR was used to confirm the screening results. Positive strains for fos genes were further subjected to whole-genome sequencing (WGS). Phenotypic screening was positive in 9/55 strains (16.4%), although genotypic detection was positive in only 3 (fosA3, fosA4 and fosA6). Thus, the prevalence of fos genes in Madrid was 5.5% (3/55). WGS data were not available for the fosA6-positive strain. One isolate with fosA3 (ST69) carried a blaCTX-M-55 gene and seven virulence genes (air, eilA, iha, iss, lpfA, sat and senB). The fosA4-positive isolate (ST4038) carried the virulence genes iss, lpfA, iroN and mchF. Both fos genes were located between two IS26 mobile elements of a plasmid. We detected the presence of different variants of plasmid-mediated fosA genes in fosfomycin-resistant E. coli strains in Madrid, Spain. Despite the few reports in Europe, it would be of interest to monitor the spread of these acquired resistance genes. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Antibiotic resistance | es_ES |
dc.subject | Escherichia coli | es_ES |
dc.subject | Fosfomycin | es_ES |
dc.subject | Urinary tract infection | es_ES |
dc.subject | fosA | es_ES |
dc.title | Prevalence, detection and characterisation of fosfomycin-resistant Escherichia coli strains carrying fosA genes in Community of Madrid, Spain. | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.identifier.pubmedID | 33757820 | es_ES |
dc.format.volume | 25 | es_ES |
dc.format.page | 137-141 | es_ES |
dc.identifier.doi | 10.1016/j.jgar.2021.02.032 | es_ES |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 2213-7173 | es_ES |
dc.relation.publisherversion | https://doi.org/10.1016/j.jgar.2021.02.032 | es_ES |
dc.identifier.journal | Journal of Global Antimicrobial Resistance | es_ES |
dc.repisalud.centro | ISCIII::Centro Nacional de Microbiología | es_ES |
dc.repisalud.institucion | ISCIII | es_ES |
dc.rights.accessRights | open access | es_ES |