Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/12529
Título
Hookworms Evade Host Immunity by Secreting a Deoxyribonuclease to Degrade Neutrophil Extracellular Traps.
Autor(es)
Bouchery, Tiffany | Moyat, Mati | Sotillo, Javier ISCIII | Silverstein, Solomon | Volpe, Beatrice | Coakley, Gillian | Tsourouktsoglou, Theodora-Dorita | Becker, Luke | Shah, Kathleen | Kulagin, Manuel | Guiet, Romain | Camberis, Mali | Schmidt, Alfonso | Seitz, Arne | Giacomin, Paul | Le Gros, Graham | Papayannopoulos, Venizelos | Loukas, Alex | Harris, Nicola L
Fecha de publicación
2020
Cita
Cell Host Microbe . 2020 Feb 12;27(2):277-289.e6.
Idioma
Inglés
Tipo de documento
journal article
Resumen
Hookworms cause a major neglected tropical disease, occurring after larvae penetrate the host skin. Neutrophils are phagocytes that kill large pathogens by releasing neutrophil extracellular traps (NETs), but whether they target hookworms during skin infection is unknown. Using a murine hookworm, Nippostrongylus brasiliensis, we observed neutrophils being rapidly recruited and deploying NETs around skin-penetrating larvae. Neutrophils depletion or NET inhibition altered larvae behavior and enhanced the number of adult worms following murine infection. Nevertheless, larvae were able to mitigate the effect of NETs by secreting a deoxyribonuclease (Nb-DNase II) to degrade the DNA backbone. Critically, neutrophils were able to kill larvae in vitro, which was enhanced by neutralizing Nb-DNase II. Homologs of Nb-DNase II are present in other nematodes, including the human hookworm, Necator americanus, which also evaded NETs in vitro. These findings highlight the importance of neutrophils in hookworm infection and a potential conserved mechanism of immune evasion.
Palabras clave
DNAse II | Nippostrongylus brasiliensis | Helminth | Hookworm | Immune-evasion | Neutrophil extracellular traps | Neutrophils
MESH
Immune Evasion | Ancylostomatoidea | Animals | Endodeoxyribonucleases | Extracellular Traps | Host-Parasite Interactions | Mice | Neutrophils | Nippostrongylus | Strongylida Infections
Versión en línea
DOI
Aparece en las colecciones
Ficheros en el ítem
- Nombre:
- HookwormsEvadeHostImmunitySupp ...
- Tamaño:
- 19.54Mb
- Formato:
- Descripción:
- Supplementary data