dc.contributor.author | Guzmán-Puche, Julia | |
dc.contributor.author | Jenayeh, Rim | |
dc.contributor.author | Perez-Vazquez, Maria | |
dc.contributor.author | Causse, Manuel | |
dc.contributor.author | Asma, Ferjani | |
dc.contributor.author | Jalel, Boukadida | |
dc.contributor.author | Oteo-Iglesias, Jesus | |
dc.contributor.author | Martínez-Martínez, Luis | |
dc.date.accessioned | 2021-03-16T19:24:34Z | |
dc.date.available | 2021-03-16T19:24:34Z | |
dc.date.issued | 2021-02-02 | |
dc.identifier.citation | J Glob Antimicrob Resist . 2021 Feb 2;24:306-310. | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/12329 | |
dc.description.abstract | There is very limited information about OXA-48-producing Klebsiella oxytoca. The aim of this study was to describe the phenotypic and molecular characterization of OXA-48-producing K. oxytoca isolates that caused an outbreak in a hospital in Tunisia.
Nineteen OXA-48-producing K. oxytoca were isolated from 2013 to 2016 in the University Hospital Farhat Hached, Sousse, Tunisia. Antibiotic susceptibility testing was performed by broth microdilution. Carbapenemase activity was investigated using the modified carbapenem inactivation method (mCIM). Phenotypic tests were also carried out to detect extended-spectrum β-lactamases. PCR was used to test for the presence of carbapenemase genes (blaIMP, blaVIM, blaNDM, blaSPM, blaAIM, blaDIM, blaGIM, blaSIM, blaKPC, blaBIC and blaOXA-48). Genetic relatedness among isolates was investigated using rep-PCR. Whole genome sequencing (WGS) was performed in three representative isolates.
mCIM was positive in all isolates. None of the isolates presented an ESBL phenotype. All strains were susceptible to cefoxitin, ceftazidime, cefepime, aztreonam, imipenem, meropenem, fluoroquinolones, aminoglycosides and colistin, and resistant to piperacillin-tazobactam, ertapenem, ticarcillin and ampicillin-sulbactam. All isolates presented the blaOXA-48 gene located in a ca. 63 kb IncL plasmid, which carried no additional resistance genes. They belonged to the new ST220.
Isolates from this study did not co-express an ESBL, which could complicate their detection in clinical laboratories. As OXA-48 has been mostly reported in K. pneumoniae there is a risk that the production of this enzyme is not suspected in the less common species K. oxytoca. These difficulties could play an important role in the hidden spread of this enzyme. | es_ES |
dc.description.sponsorship | This research was funded in part by grants PI14/01911 (to L.M.-M.), REIPI RD16/0016/0008 and REIPI RD16CIII/0004/0002, all from Instituto de Salud Carlos III, Ministerio de Economía y Competitividad, and co-financed by the European Development Regional Fund “A Way to Achieve Europe” ERDF. This study did not receive any specific grant from commercial or not-for-profit sectors. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | IncL | es_ES |
dc.subject | Klebsiella oxytoca | es_ES |
dc.subject | OXA-48 | es_ES |
dc.title | Characterization of OXA-48-producing Klebsiella oxytoca isolates from a hospital outbreak in Tunisia. | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.identifier.pubmedID | 33545421 | es_ES |
dc.format.volume | 24 | es_ES |
dc.format.page | 306-310 | es_ES |
dc.identifier.doi | 10.1016/j.jgar.2021.01.008 | es_ES |
dc.contributor.funder | Instituto de Salud Carlos III | |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | |
dc.contributor.funder | Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) | |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 2213-7173 | es_ES |
dc.relation.publisherversion | https://doi.org/10.1016/j.jgar.2021.01.008 | es_ES |
dc.identifier.journal | Journal of global antimicrobial resistance | es_ES |
dc.repisalud.centro | ISCIII::Centro Nacional de Microbiología | es_ES |
dc.repisalud.institucion | ISCIII | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/PI14/01911 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/REIPI RD16/0016/0008 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/REIPI RD16CIII/0004/0002 | es_ES |
dc.rights.accessRights | open access | es_ES |