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dc.contributor.authorFormenti, Fabio
dc.contributor.authorTa Tang, Thuy-Huong 
dc.contributor.authorTamarozzi, Francesca
dc.contributor.authorSilva, Ronaldo
dc.contributor.authorLa Marca, Giulia
dc.contributor.authorPajola, Barbara
dc.contributor.authorPiubelli, Chiara
dc.contributor.authorPerandin, Francesca
dc.contributor.authorRubio Muñoz, Jose Miguel 
dc.contributor.authorEscolar, Eva Marina
dc.contributor.authorBisoffi, Zeno
dc.contributor.authorGobbi, Federico
dc.date.accessioned2021-03-16T11:27:44Z
dc.date.available2021-03-16T11:27:44Z
dc.date.issued2021-04
dc.identifier.citationActa Trop. 2021 Apr;216:105838.es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/12284
dc.description.abstractInfections with the filarial nematodes Loa loa and Mansonella perstans are among the most neglected filarial infections. L. loa is endemic in 11 countries of Central and West Africa and loiasis is estimated to affect about 20 million people. M. perstans infection is widespread in more than 30 countries of sub-Saharan Africa. Due to the difficulty in diagnosing loiasis and M. perstans mansonellosis on a clinical basis, the diagnosis of infection with L. loa and M. perstans relies on laboratory techniques. Definitive diagnosis is based on the detection, identification, and quantification of circulating microfilariae (mf) by microscopy of concentrated blood. However, this is impractical for screening purposes as it requires expert laboratory personnel, considerable blood manipulation, and is time consuming, especially for the final issue of negative result reports, which are very common in the population visited outside endemic areas. The aim of the current work is the preliminary evaluation of the performance of the in-house real-time PCR described by Ta and colleagues compared to the routine microscopic approach for the screening of filarial infections in the clinical setting outside endemic areas, using samples from patients accessing the dedicated outpatient clinics for migrants and travelers of a reference centre for tropical diseases in Northern Italy.es_ES
dc.description.sponsorshipThis work was supported by the Italian Ministry of Health “Fondi Ricerca Corrente – Progetto L3P2”.es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectFilarial infectionses_ES
dc.subjectLoa loaes_ES
dc.subjectMansonella perstanses_ES
dc.subjectMolecular diagnosises_ES
dc.subjectReal-timees_ES
dc.subjectPCRes_ES
dc.subjectITS1es_ES
dc.subjectMicroscopyes_ES
dc.titlePreliminary comparison between an in-house real-time PCR vs microscopy for the diagnosis of Loa loa and Mansonella perstanses_ES
dc.typeArtículoes_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID33484727es_ES
dc.format.volume216es_ES
dc.format.page105838es_ES
dc.identifier.doi10.1016/j.actatropica.2021.105838es_ES
dc.contributor.funderMinistero della Salute (Italia)es_ES
dc.description.peerreviewedes_ES
dc.identifier.e-issn1873-6254es_ES
dc.relation.publisherversionhttps://doi.org/10.1016/j.actatropica.2021.105838es_ES
dc.identifier.journalActa tropicaes_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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Atribución 4.0 Internacional
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