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dc.contributor.author | Perez-Vazquez, Maria | |
dc.contributor.author | Oteo-Iglesias, Jesus | |
dc.contributor.author | Sola-Campoy, Pedro Juan | |
dc.contributor.author | Carrizo-Manzoni, Hugo | |
dc.contributor.author | Bautista, Veronica | |
dc.contributor.author | Lara Fuella, Noelia | |
dc.contributor.author | Aracil, Belen | |
dc.contributor.author | Alhambra, Almudena | |
dc.contributor.author | Martínez-Martínez, Luis | |
dc.contributor.author | Campos, Jose | |
dc.date.accessioned | 2021-01-08T08:18:42Z | |
dc.date.available | 2021-01-08T08:18:42Z | |
dc.date.issued | 2019 | |
dc.identifier.citation | Antimicrob Agents Chemother . 2019 May 24;63(6):e02529-18. | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/11581 | |
dc.description.abstract | There is little information about carbapenemase-producing (CP) Klebsiella oxytoca, an important nosocomial pathogen. We characterized CP K. oxytoca isolates collected from different Spanish hospitals between January 2016 and October 2017. During the study period, 139 nonduplicate CP K. oxytoca isolates were identified; of these, 80 were studied in detail. Carbapenemase and extended-spectrum β-lactamase genes were identified by PCR and sequencing. Genetic relatedness was studied by pulsed-field gel electrophoresis (PFGE). Whole-genome sequencing (WGS), carried out on 12 representative isolates, was used to identify the resistome, to elucidate the phylogeny, and to determine the plasmids harboring carbapenemase genes. Forty-eight (60%) isolates produced VIM-1, 30 (37.5%) produced OXA-48, 3 (3.7%) produced KPC-2, 2 (2.5%) produced KPC-3, and 1 (1.2%) produced NDM-1; 4 isolates coproduced two carbapenemases. By PFGE, 69 patterns were obtained from the 80 CP K. oxytoca isolates, and four well-defined clusters were detected: cluster 1 consisted of 11 OXA-48-producing isolates, and the other three clusters included VIM-1-producing isolates (5, 3, and 3 isolates, respectively). In the 12 sequenced isolates, the average number of acquired resistance genes was significantly higher in VIM-1-producing isolates (10.8) than in OXA-48-producing isolates (2.3). All 12 isolates had chromosomally encoded genes of the blaOXY-2 genotype, and by multilocus sequence typing, most belonged to sequence type 2 (ST2). Carbapenemase genes were carried by IncL, IncHI2, IncFII, IncN, IncC, and IncP6 plasmid types. The emergence of CP K. oxytoca was principally due to the spread of VIM-1- and OXA-48-producing isolates in which VIM-1- and OXA-48 were carried by IncL, IncHI2, IncFII, and IncN plasmids. ST2 and the genotype blaOXY-2 predominated among the 12 sequenced isolates. | es_ES |
dc.description.sponsorship | This work was supported by Plan Nacional de I+D+i 2013-2016 and the Instituto de Salud Carlos III, Subdirección General de Redes y Centros de Investigación Cooperativa, Ministerio de Ciencia, Innovación y Universidades, Spanish Network for Research in Infectious Diseases (REIPI RD16CIII/0004/0002, RD16/0016/0008), cofinanced by the European Development Regional Fund ERDF (A way to achieve Europe) operative program Intelligent Growth 2014-2020. This work was also supported by a grant from the Instituto de Salud Carlos III (grant number MPY 1135/16) and by the Antibiotic Resistance Surveillance Program of the Centro Nacional de Microbiología (Instituto de Salud Carlos III, Ministerio de Economía y Competitividad) of Spain. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | American Society for Microbiology (ASM) | es_ES |
dc.type.hasVersion | AM | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | * |
dc.subject | Klebsiella oxytoca | es_ES |
dc.subject | Carbapenemases | es_ES |
dc.subject | cgMLST | es_ES |
dc.subject | Plasmids | es_ES |
dc.subject | Whole-genome sequencing | es_ES |
dc.subject.mesh | Bacterial Proteins | es_ES |
dc.subject.mesh | Drug Resistance, Multiple, Bacterial | es_ES |
dc.subject.mesh | Electrophoresis, Gel, Pulsed-Field | es_ES |
dc.subject.mesh | Humans | es_ES |
dc.subject.mesh | Klebsiella oxytoca | es_ES |
dc.subject.mesh | Microbial Sensitivity Tests | es_ES |
dc.subject.mesh | Multilocus Sequence Typing | es_ES |
dc.subject.mesh | Plasmids | es_ES |
dc.subject.mesh | Spain | es_ES |
dc.subject.mesh | beta-Lactamases | es_ES |
dc.title | Characterization of Carbapenemase-Producing Klebsiella oxytoca in Spain, 2016-2017. | es_ES |
dc.type | research article | es_ES |
dc.rights.license | Atribución-NoComercial-CompartirIgual 4.0 Internacional | * |
dc.identifier.pubmedID | 30936106 | es_ES |
dc.format.volume | 63 | es_ES |
dc.format.number | 6 | es_ES |
dc.identifier.doi | 10.1128/AAC.02529-18 | es_ES |
dc.contributor.funder | Instituto de Salud Carlos III | |
dc.contributor.funder | RETICS-Investigación en Patología Infecciosa (REIPI-ISCIII) (España) | |
dc.contributor.funder | Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) | |
dc.description.peerreviewed | Sí | es_ES |
dc.relation.publisherversion | https://doi.org/10.1128/AAC.02529-18 | es_ES |
dc.identifier.journal | Antimicrobial agents and chemotherapy | es_ES |
dc.repisalud.centro | ISCIII::Centro Nacional de Microbiología | es_ES |
dc.repisalud.institucion | ISCIII | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/REIPI RD16CIII/0004/0002 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/RD16/0016/0008 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/MPY 1135/16 | es_ES |
dc.rights.accessRights | open access | es_ES |