Please use this identifier to cite or link to this item:http://hdl.handle.net/20.500.12105/11464
Epidemiology, molecular characterisation and antimicrobial susceptibility of Neisseria gonorrhoeae isolates in Madrid, Spain, in 2016.
Guerrero-Torres, M D | Menéndez, MB | Guerras, CS | Tello, E | Ballesteros, J | Clavo, P | Puerta, T | Vera, M | Ayerdi, O | Carrio, J C | Mozo, I | Del Romero, J | Vazquez-Moreno, Julio Alberto ISCIII | Abad, Raquel ISCIII
Epidemiol Infect . 2019 Sep 24;147:e274.
With the aim to elucidate gonococcal antimicrobial resistance (AMR)-risk factors, we undertook a retrospective analysis of the molecular epidemiology and AMR of 104 Neisseria gonorrhoeae isolates from clinical samples (urethra, rectum, pharynx and cervix) of 94 individuals attending a sexually transmitted infection clinic in Madrid (Spain) from July to October 2016, and explored potential links with socio-demographic, behavioural and clinical factors of patients. Antimicrobial susceptibility was determined by E-tests, and isolates were characterised by N. gonorrhoeae multi-antigen sequence typing. Penicillin resistance was recorded for 15.4% of isolates, and most were susceptible to NG-MASTtetracycline, cefixime and azithromycin; a high incidence of ciprofloxacin resistance (~40%) was found. Isolates were grouped into 51 different sequence types (STs) and 10 genogroups (G), with G2400, ST5441, ST2318, ST12547 and G2992 being the most prevalent. A significant association (P = 0.015) was evident between HIV-positive MSM individuals and having a ciprofloxacin-resistant strain. Likewise, a strong association (P = 0.047) was found between patient age of MSM and carriage of isolates expressing decreased susceptibility to azithromycin. A decrease in the incidence of AMR gonococcal strains and a change in the strain populations previously reported from other parts of Spain were observed. Of note, the prevalent multi-drug resistant genogroup G1407 was represented by only three strains in our study, while the pan-susceptible clones such as ST5441, and ST2318, associated with extragenital body sites were the most prevalent.
Drug Resistance, Bacterial | Genotype | Adolescent | Adult | Disk Diffusion Antimicrobial Tests | Female | Gonorrhea | Humans | Incidence | Male | Middle Aged | Molecular Epidemiology | Multilocus Sequence Typing | Neisseria gonorrhoeae | Retrospective Studies | Risk Factors | Spain | Young Adult
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