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dc.contributor.authorGonzález-Serrano, Lydia
dc.contributor.authorMuñoz-Algarra, María
dc.contributor.authorGonzalez-Sanz, Ruben 
dc.contributor.authorPortero-Azorín, María Francisca
dc.contributor.authorAmaro, María José
dc.contributor.authorHigueras, Purificación
dc.contributor.authorCabrerizo, Maria 
dc.date.accessioned2020-11-17T13:01:44Z
dc.date.available2020-11-17T13:01:44Z
dc.date.issued2020
dc.identifier.citationJ Clin Virol . 2020 Jul;128:104420es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/11355
dc.description.abstract. Viral infections are recognized as the most common cause of acute gastroenteritis (AGE). Virus detection by immune analytical methods is recommended for diagnosis because of its simplicity and low cost. . Two commercial immunochromatographic (ICG) techniques (Materlab) for rapid detection of rotavirus/adenovirus and norovirus respectively, were evaluated by comparison to the results obtained using PCR methods. In addition, clinical and epidemiologic characteristics of AGE infections have been described. . A total of 100 faecal samples collected from patients with AGE (84% children) admitted into a Spanish Hospital between February and July 2018, were studied for rotavirus-A, adenovirus and norovirus GI/GII by the ICG tests as well as by PCR and sequencing. Other enteric viruses (enterovirus and astrovirus) were investigated by PCR methods. Gastrointestinal bacteria and parasites were also tested. . Evaluated ICG tests yielded high specificity (>97%). Sensitivity values were high for rotavirus/adenovirus (>80%) but lower for norovirus (57%). Overall, and taking into account coinfections, viruses (32%), bacteria (14%) and parasites (1%) could be detected. Rotavirus-A were the most frequently identified viruses (16%), followed by enterovirus (12%), norovirus (4%), adenovirus 41 (4%) and astrovirus (1%). In five vaccinated children, a rotavirus was detected. . ICG technique is a useful tool for the routine diagnosis of AGE infections at hospital, but for surveillance and epidemiological studies, it is needed the use of amplification and sequencing methods, which also allow monitoring of new strains or variants emergence. In this study, an etiological pathogen was determined only in 44% of samples.es_ES
dc.language.isoenges_ES
dc.publisherElsevier es_ES
dc.type.hasVersionAMes_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectAdenoviruses_ES
dc.subjectImmunochromatographic testes_ES
dc.subjectNoroviruses_ES
dc.subjectPCRes_ES
dc.subjectRotaviruses_ES
dc.titleViral gastroenteritis in hospitalized patients: Evaluation of immunochromatographic methods for rapid detection in stool samples.es_ES
dc.typejournal articlees_ES
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.identifier.pubmedID32454428es_ES
dc.format.volume128es_ES
dc.format.page104420es_ES
dc.identifier.doi10.1016/j.jcv.2020.104420es_ES
dc.description.peerreviewedes_ES
dc.identifier.e-issn1873-5967
dc.relation.publisherversionhttps://doi.org/10.1016/j.jcv.2020.104420es_ES
dc.identifier.journalJournal of clinical virology : the official publication of the Pan American Society for Clinical Virologyes_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.rights.accessRightsopen accesses_ES


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Este Item está sujeto a una licencia Creative Commons: Attribution-NonCommercial-NoDerivatives 4.0 Internacional