Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/11291
Título
A Founder Effect Led Early Sars-Cov-2 Transmission In Spain
Autor(es)
Díez-Fuertes, Francisco ISCIII | Iglesias-Caballero, Maria ISCIII | García-Pérez, Javier ISCIII | Monzon-Fernandez, Sara ISCIII | Jimenez Sancho, Maria Pilar ISCIII | Varona Fernandez, Sarai ISCIII | Cuesta de la Plaza, Isabel ISCIII | Zaballos, Ángel ISCIII | Jimenez, Mercedes ISCIII | Checa, Laura | Pozo Sanchez, Francisco ISCIII | Perez-Olmeda, Mayte ISCIII | Thomson, Michael M ISCIII | Alcamí, José ISCIII | Casas Flecha, Inmaculada ISCIII
Fecha de publicación
2021-01
Cita
J Virol. 2021 Jan 13;95(3):e01583-20.
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Tipo de documento
research article
Resumen
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) whole-genome analysis has identified five large clades worldwide which emerged in 2019(19A and 19B) and in 2020 (20A, 20B, and 20C). This study aimed to analyze the dif-fusion of SARS-CoV-2 in Spain using maximum-likelihood phylogenetic and Bayesianphylodynamic analyses. The most recent common ancestor (MRCA) of the SARS-CoV-2 pandemic was estimated to have emerged in Wuhan, China, around 24 No-vember 2019. Phylogenetic analyses of the first 12,511 SARS-CoV-2 whole-genomesequences obtained worldwide, including 290 from 11 different regions of Spain, re-vealed 62 independent introductions of the virus in the country. Most sequencesfrom Spain were distributed in clades characterized by a D614G substitution in the Sgene (20A, 20B, and 20C) and an L84S substitution in ORF8 (19B) with 163 and 118sequences, respectively, with the remaining sequences branching in 19A. A total of110 (38%) sequences from Spain grouped in four different monophyletic clusters ofclade 20A (20A-Sp1 and 20A-Sp2) and 19B clade (19B-Sp1 and 19B-Sp2) along withsequences from 29 countries worldwide. The MRCAs of clusters 19A-Sp1, 20A-Sp1,19A-Sp2, and 20A-Sp2 were estimated to have occurred in Spain around 21 and 29January and 6 and 17 February 2020, respectively. The prevalence of clade 19B inSpain (40%) was by far higher than in any other European country during the firstweeks of the epidemic, probably as a result of a founder effect. However, this vari-ant was replaced by G614-bearing viruses in April.In vitroassays showed an en-hanced infectivity of pseudotyped virions displaying the G614 substitution comparedwith those having D614, suggesting a fitness advantage of D614G.
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